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| 烧骨DNA检验技术的研究 | |
| 引用本文： | Ye J,Ji AQ,Zhao XC. 烧骨DNA检验技术的研究[J]. 法医学杂志, 2004, 20(3): 155-159 |
| 作者姓名： | Ye J Ji AQ Zhao XC |
| 作者单位： | 1. 公安部第二研究所,北京,100038 2. 菏泽市公安局,山东,荷泽,274039 3. 中国人民公安大学,北京,100038 |
| 摘 要： | 目的解决陈旧性骨骼和烧骨DNA检验难题。方法研究建立了CTAB法裂解提取DNA,再用磁珠纯化得到的DNA提取液进行STR复合扩增检验。结果实验结果及实际检案显示研究所建立的骨DNA提取方法能较好地去除DNA扩增抑制物,得到高质量的DNA模板。结论本研究所建立的烧骨DNA检验方法其识别率为10×10-12,达到个人同一认定的目的,在解决实际工作中杀人焚尸案、火灾、爆炸等恶性案件和事故中有重要的作用。 |
| 关 键 词： | 十六烷基三甲基溴化胺(CTAB) 陈旧性骨骼和烧骨DNA 磁珠纯化 法医DNA检验 荧光标记STR复合扩增 |
| 文章编号： | 1004-5619(2004)03-0155-05 |
| 修稿时间： | 2003-08-21 |
Study of DNA identification in burned bones |
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| Ye Jian,Ji An-quan,Zhao Xing-chun. Study of DNA identification in burned bones[J]. Journal of Forensic Medicine, 2004, 20(3): 155-159 | |
| Authors: | Ye Jian Ji An-quan Zhao Xing-chun |
| Affiliation: | Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China. yejian_21@sina.com.cn |
| Abstract: | Objective For the purpose of solving a problems of DNA testing of burned bones.Methods We present a novel strategy to obtain DNA from burned bones based on the use of cetyltrimethylammoni-um bromide(CTAB)lysis buffer and isoamyl alcohol-chlorophorm extraction with subsequent DNA purifi-cation using the DNA IQTMSystem.Results The methods were found to be effective in removing the PCR inhibitors from the burned bone.Then the extracted DNA was successfully genotyped by using the flores-cence labeling STR multiplex method.Conclusion The results of this research will assist forensic scien-tists in the identification of DNA from victims whose bodies underwent significant trauma or burning,pre-cluding the utilization of traditional forensic DNA identification techniques. |
| Keywords: | cetyltrimethyl ammonium bromide(CTAB) collagen aged bones and burned bones DNA DNA IQTMsystem purification forensic DNA examination florescence labeling STR multiplex |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |