Molecular identification of forensically important fly species in Spain using COI barcodes |
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| Institution: | 1. Departamento de Zoología y Antropología Física, Campus Mare Nostrum, Universidad de Murcia, Campus de Espinardo, 30100 Murcia, Spain;2. Departamento de Biología Animal, Edafología y Geología, Universidad de La Laguna, 38200 La Laguna, Tenerife, Canary Islands, Spain;1. School of Criminal Justice, University of Lausanne, Lausanne, Switzerland;2. University of Québec at Trois-Rivières, Trois-Rivières, Canada;3. Laboratoire de recherche en criminalistique, Trois-Rivières, Canada;1. Department of Human Biology, University of Cape Town, Observatory, Cape Town, South Africa;2. Department of Pathology, University of Cape Town, Observatory, Cape Town, South Africa;1. Departamento de Ciencias de la Vida, Universidad de Alcalá, 28805, Alcalá de Henares, Madrid, Spain;2. Department of Biology, University of Ottawa, 30 Marie-Curie Private, Ottawa, Ontario, K1N 6N5, Canada;3. Instituto Universitario de Investigación en Ciencias Policiales, 28801, Alcalá de Henares, Spain;1. Department of Forensic Medicine, Soochow University, Ganjiang East Road, Suzhou, China;2. Taikang Xianlin Drum Tower Hospital, Nanjing, China;3. School of Forensic Medicine, Wannan Medical College, China |
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| Abstract: | Species identification with DNA barcodes has been proven to be effective on different organisms and, particularly, has become a routinely used and quite accurate tool in forensic entomology to study necrophagous Diptera species. In this study, we analysed 215 specimens belonging to 42 species of 17 genera, from 9 different Diptera families. Flies were collected in 39 Spanish localities of the Iberian Peninsula sampled across three years in the four seasons. Intraspecific variation ranged from 0 to 2.46% whereas interspecific variation fluctuated from 3.07 to 14.59%, measuring 651 pb of the cytochrome oxidase subunit one (COI) gene. Neighbour-Joining analysis was carried out to investigate the molecular identification capabilities of the barcoding region, recovering almost all species as distinct monophyletic groups. The species groupings were generally consistent with morphological and molecular identifications. This work, which is the first with this intensive and extensive sampling in this area, shows that the COI barcode is an appropriate marker for unambiguous identification of forensically important Diptera in Spain. |
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| Keywords: | Cytochrome oxidase subunit one (COI) Forensic entomology Iberian Peninsula Necrophagous Diptera Species identification |
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