Use of RGB values in the Periodic Acid-Schiff color test to determine the presence of vaginal fluid |
| |
Affiliation: | 1. Division of Cardiology, Lehigh Valley Health Network, Allentown, PA, United States;2. Department of Medicine, Sinai Hospital of Baltimore, Johns Hopkins University, Baltimore, MD, United States;3. Department of Medicine, Division of Cardiology, Einstein Healthcare Network, Philadelphia, PA, United States;4. Helmsley Electrophysiology Center, Mount Sinai School of Medicine, New York, NY, United States;5. Texas Cardiac Arrhythmia Institute at St. David''s Medical Center, Austin, TX, United States |
| |
Abstract: | This study demonstrates how RGB color values from microscopic smears stained with the Periodic Acid-Schiff reagent under standardized microscopy conditions can be used to indicate the presence of vaginal secretions. Based on data obtained in the study, a numeric threshold determined from the sum of separate values for red, blue and green was determined to differentiate vaginal-based samples with other body fluids. Using this threshold, 55 of 57 vaginal-based samples tested positive for the presence of vaginal secretion. Conversely, 27 of 29 smears prepared from other body fluids yielded negative results. However, when graphing RGB sum values against a calculated RGB integer no overlap in data was obtained between all vaginal-based samples and other body fluid samples, clearly differentiating them. One-way ANOVA testing with a 95% confidence interval indicated that vaginal samples from different age groups showed no difference in RGB sum values. Similarly, the location that vaginal swabs were collected (from the outside of a condom or a vaginal swab) also showed no statistical difference using one-way ANOVA at 95% confidence. Furthermore, refrigerated test swabs aged up to 15 months showed no demonstrable differences. Pair-wise t-testing using RGB sum values, however, did show significant differences between vaginal samples and all other body fluids tested. Finally, the method successfully differentiated between pre-and post-coital penile swabs and finger swabs taken before and after digital vaginal penetration in anecdotal comparisons using the method. |
| |
Keywords: | Vaginal secretion identification Periodic Acid Shiff reagent RGB color system |
本文献已被 ScienceDirect 等数据库收录! |
|