A survey of bacteria associated with various life stages of primary colonizers: Lucilia sericata and Phormia regina |
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| Affiliation: | 1. Integrative Life Sciences, Virginia Commonwealth University, Richmond, VA, United States;2. Department of Forensic Science, Virginia Commonwealth University, Richmond, VA, United States;3. Department of Chemistry, University of Virginia, Charlottesville, VA, United States;1. Department of Forensic Medicine, School of Basic Medical Sciences, Chongqing Medical University, Chongqing, China;2. Key Laboratory of Evidence Identification in Universities of Shandong Province, Shandong University of Political Science and Law, Jinan, China;3. Chongqing Key Laboratory of Vehicle/Biological Crash Security, Research Institute for Traffic Medicine, Daping Hospital, Third Military Medical University, Chongqing, China;1. Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy;2. The BioRobotics Institute, Sant’Anna School of Advanced Studies, viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy;1. Department of Medical Entomology and Vector Control, Faculty of Health, Hormozgan University of Medical Sciences, Bandar Abbas, Iran;2. Social Determinants in Health Promotion Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran;3. Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran;4. Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;5. Department of Biostatistics & Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran;6. Department of Pediatrics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran;1. Department of Entomology, Texas AM University – 2475 TAMU, College Station, TX 77843, USA;2. Department of Animal Evolution and Biodiversity, University of Goettingen – Untere Karspuele 2, 37073 Goettingen, Germany |
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| Abstract: | Blow flies are common primary colonizers of carrion, play an important role in the transfer of microbes between environments, and serve as a vector for many human pathogens. While some investigation has begun regarding the bacteria associated with different life stages of blow flies, a well replicated study is currently not available for the majority of blow flies. This study investigated bacteria associated with successive life stages of blow fly species Lucilia sericata and Phormia regina. A total of 38 samples were collected from four true replicates of L. sericata and P. regina. Variable region four (V4) of 16S ribosomal DNA (16S rDNA) was amplified and sequenced on MiSeq FGx sequencing platform using universal 16S rDNA primers and dual-index sequencing strategy. Bacterial communities associated with different life stages of L. sericata and P. regina didn't differ significantly from each other. In both blow fly species, Bacilli (e.g., Lactococcus) and Gammaproteobacteria (e.g., Providencia) constituted >95% of all bacterial classes across all life stages. At the genus level, Vagococcus and Leuconostoc were present at relatively high abundances in L. sericata whereas Yersinia and Proteus were present at comparatively high abundances in P. regina. Overall, information on bacterial structures associated with various life stages of blow flies can help scientists in better understanding or management of vector-borne pathogen dispersal and in increasing the accuracy of microbial evidence based postmortem interval (PMI) prediction models. |
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| Keywords: | Decomposition Insect microbiome Blow flies 16S rDNA Medical and veterinary entomology |
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