| 实验性心肌缺血的早期组织化学和超微结构变化 |
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| 引用本文： | 张永亮,孙慧宽,李灵芝,董建国. 实验性心肌缺血的早期组织化学和超微结构变化[J]. 中国法医学杂志, 1990, 0(4) |
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| 作者姓名： | 张永亮 孙慧宽 李灵芝 董建国 |
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| 作者单位： | 内蒙古医学院法医学教研室 呼和浩特市(张永亮，孙慧宽，李灵芝)，内蒙古医学院法医学教研室 呼和浩特市(董建国) |
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| 摘 要： | 作者们应用酶组织化学和电子显微镜技术,对实验性家兔心肌缺血的早期改变进行研究。发现当心肌缺血15min,PAS、PPr、分枝酶即呈阴性,SDH、NADPHD、GDH、ATPase、CCo 活性降低,且随缺血时间延长,缺血范围逐渐增大,酶活性逐渐降低,MAO、ACP、ANAE 活性在缺血早期无明显改变。电镜观察,缺血早期的主要改变是线粒体肿胀、增大、嵴断裂溶解,基质内出现致密颗粒。上述结果有助于急性心肌缺血的早期诊断及缺血时间判定。
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| 关 键 词： | 实验性心肌缺血 酶组织化学 超微结构 |
EARLY HISTOCHEMICAL AND ULTRASTRUCTURAL ALTERATIONS IN THE EXPERIMENTAL ISCHEMIC MYOCARDIUM |
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| Zhang Yongliang Sun Huikuan Li Lingzhi Dong Jianguo. EARLY HISTOCHEMICAL AND ULTRASTRUCTURAL ALTERATIONS IN THE EXPERIMENTAL ISCHEMIC MYOCARDIUM[J]. Chinese Journal of Forensic Medicine, 1990, 0(4) |
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| Authors: | Zhang Yongliang Sun Huikuan Li Lingzhi Dong Jianguo |
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| Affiliation: | Zhang Yongliang Sun Huikuan Li Lingzhi Dong Jianguo Department of Forensic Medicine.Inner Mongolian medical College Hnhehaote |
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| Abstract: | Early ultrastructural and histochemical changes in the myocardium following co-ronary artery occlusion in the rabbit were studied by TEM and histochemical me-thods.At 15 rain of ischemia,PAS,PPr and branching enzyme were negative,and de- hydrogenase(SDH,NADPHD,GDH),ATPase,as well as CCo were diminished.Progr-essively with the prolongation of the time after ischemia,the activities of enzymesreduced while the area of ischemia enlarged.Significant changes of MAO,ACP andANAE could notbe recognized at early ischemia.The main ultrastructural altera-tions of early ischemia showed swelling of mitochondria,disorganization of cristaeand the appearance of amorphous densities in the matrix.The authors think thatthe histochemical and ultrastructural alterations are helpful for early diagnosisand timing of early acute myocardial ischemia. |
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| Keywords: | Experimental myocardial ischemia Enzyme-histochemistry Ultrastructure |
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