| DNA数据库建设中批量样本直接扩增检验法的应用 |
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| 引用本文： | 刘超,冯冬亮,刘长晖.DNA数据库建设中批量样本直接扩增检验法的应用[J].中国法医学杂志,2010,25(2):79-81. |
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| 作者姓名： | 刘超 冯冬亮 刘长晖 |
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| 作者单位： | 广州市刑事科学技术研究所,广东,广州,510030 |
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| 摘 要： | 目的研究批量样本直接扩增法在DNA数据库建设中的应用。方法打孔机打取血滤纸600份,剪取芝麻大小的口腔拭子300份,放入96孔扩增板,加入扩增试剂后直接扩增,并对其中200份血滤纸用磁珠法,100份口腔拭子用96孔板Chelex-100法,经DNA提取后扩增检验进行比较。结果血滤纸采用直接扩增法及磁珠法STR检测成功率均为100%;口腔拭子采用直接扩增法的成功率为95%,采用96孔板Chelex-100法的成功率为94%。结论血滤纸和口腔拭子通过直接扩增均能获得很好的DNA分型结果,该方法省时省力,可用于DNA数据库建设。
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| 关 键 词： | 法医物证学 DNA数据库 直接扩增法 磁珠法 Chelex-100法 |
Application of direct amplification on establishing DNA database |
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| LIU Chao,FENG Dongliang,LIU Changhui.Application of direct amplification on establishing DNA database[J].Chinese Journal of Forensic Medicine,2010,25(2):79-81. |
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| Authors: | LIU Chao FENG Dongliang LIU Changhui |
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| Institution: | LIU Chao;FENG Dongliang;LIU Changhui/Guangzhou Institute of Criminal Science and Technology;Guangzhou 510030;China |
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| Abstract: | Objective To develop direct amplification of DNA from samples of DNA database.Methods 600 punches from blood samples and 300 cuts from buccal samples were added to 96-well plates for direct amplification.200 blood samples and 100 buccal samples were randomly collected,and the blood samples were extracted with magnetic beads method while the buccal samples with Chelex-100 method.Results For blood samples,success rates of direct amplification and magnetic beads method are 100%.For buccal samples,success rate ... |
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| Keywords: | forensic biological evidence DNA database direct amplification magnetic beads method Chelex-100 method |
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