合成大麻素类新精神活性物质AB-CHMINACA的体内与体外代谢研究

目的 通过UPLC-HRMS检测分析体外人肝微粒体模型中合成大麻素类新精神活性物质AB-CHMINACA的代谢物并与一例真实滥用者的尿液样本进行对比,从而对体外人肝微粒体模型预测体内代谢物的一致性进行评价研究.方法 通过建立体外人肝微粒体孵育模型模拟人体体内代谢过程,尿液样本经简单的乙腈沉淀蛋白后利用高分辨质谱(HRM...

In Vitro and in Vivo Human Metabolism of the Synthetic Cannabinoid New Psychoactive Substance AB-CHMINACA

Objective To detect and analyze the in vitro metabolites of AB-CHMINACA which were generated in the in vitro human liver microsomes model system by Ultra Performance Liquid Chromatography(UPLC)coupled to High Resolution Mass Spectrum(HR-MS).And then compare the results with the urine sample with a real abuser.Methods To simulate the metabolic process in human body by establishing the in vitro human liver microsomes model,and then determine and elaborate the reaction points and pathways of metabolism by HR-MS.Results AB-CHMINACA generated 10 metabolites in the in vitro human liver microsomes model totally.Carboxamide hydrolysis,hydroxylation on the methylpropyl moiety and the combination of N-dealkylation and carboxamide hydrolysis were its main metabolic pathways.Carboxamide hydrolysis metabolite M1,hydroxylation on the methylpropyl moiety metabolite M4 and the combination of N-dealkylation and carboxamide hydrolysis metabolite M10 also appeared in the urine sample of a AB-CHMINACA abuser.Conclusion The method is fast and efficient,and it can predict the in vivo metabolites to a certain extent.So we suggest that the carboxamide hydrolysis metabolite and hydroxylation on the methylpropyl moiety metabolite can be used as biomarkers for routine analysis of urine samples.