LIFE BEYOND LIFE – An Easy Way to Derive Lung Fibroblasts from Cadavers |
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| Authors: | Sophie Colomb MD PhD Corinne Bareil PhD Eric Baccino MD PhD Jean‐Philippe Berthet MD PhD Lucie Knabe PhD Isabelle Vachier PhD Arnaud Bourdin MD PhD |
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| Institution: | 1. Emergency Pole, Forensic Medicine Department, Montpellier University Hospital, Montpellier, France;2. Molecular Genetics Laboratory – IURC, Montpellier University Hospital, Montpellier, France;3. Heart‐Lung Pole, Cardiac, Thoracic and Vascular Surgery, Montpellier University Hospital, Montpellier, France;4. Heart‐Lung Pole, Department of Lung Diseases and Addictology, Montpellier University Hospital, Montpellier, France |
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| Abstract: | Several protocols have illustrated the possibility of deriving cells, such as fibroblasts, from different organs. These techniques generally concern organs sampled from living persons, but have already been described for cadavers, especially concerning the skin and tendons. We present, for the first time, an easy way to derive pulmonary fibroblasts from a lung tissue sampled from a cadaver and directly culture plated. The fibroblast output was checked daily. We obtained lung fibroblasts from 3 (60%) cadavers and 2 (100%) living persons. The fibroblast output took about 3 days for cells from living persons and took up to 39 days for those from cadavers. We did not clearly identify any parameters that could explain these differences. Nevertheless, these derived cells had the same features as the source cells, especially in terms of morphology and proliferation, and could potentially be used in different research domains such as forensic or regeneration medicine. |
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| Keywords: | forensic science explant fibroblast lung senescence forensic autopsy postmortem delay |
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