液相色谱-质谱联用测定血中氟乙酸类鼠药 |
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引用本文: | 陈学国,朱昱,张婷,许英健. 液相色谱-质谱联用测定血中氟乙酸类鼠药[J]. 刑事技术, 2010, 0(6): 22-24 |
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作者姓名: | 陈学国 朱昱 张婷 许英健 |
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作者单位: | 中国刑警学院法医系,辽宁沈阳110854 |
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摘 要: | 目的建立血中氟乙酸类鼠药液相色谱-电喷雾离子阱质谱分析方法。方法血样经甲醇沉淀蛋白后离心,上清液用氮气吹干,流动相定容过滤膜,滤液直接进行液相色谱-电喷雾离子阱质谱联用分析。结果血中氟乙酸根在0.050μg/mL-2.0μg/mL之间具有良好的线性关系,最低检出限为0.020μg/mL。结论本文建立的方法快速、灵敏、操作简便,适用于刑事案件中该类氟乙酸类鼠药的快速检验,具有一定的实用价值。
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关 键 词: | 法医毒物分析 氟乙酸根 液相色谱-质谱 血 鼠药 |
Determination of monofluoroacetate in human blood by liquid chromatography-mass spectrometry |
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Affiliation: | CHEN Xue-guo,ZHU Yu,ZHANG Ting(Department of Forensic Medicine,China Criminal Police College,Shenyang 110035,China |
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Abstract: | To establish an analysis method of monofluroacetate in human blood by using liquid chromatography and ion trap electro-spray mass spectrometry.Methods The human blood samples were wiped off protein with methanol,the supernatant was evaporated under nitrogen.The residues were reconstituted in 0.50 mL mobile phase and filtered through a membrane,and then were directly analyzed by liquid chromatography and ion trap electro-spray mass spectrometry.Results The calibration curves showed good linearity in rang of 0.050 μg/mL-2.0 μg/mL and the limit of detection was 0.020 μg/mL,respectively.Conclusion The method was sensitive and easy to operate.It can be widely applied in the identification of monofluroacetate in casework. |
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Keywords: | forensic toxicology monofluroacetate liquid chromatography-mass spectrometry blood rodenticide |
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