| phyA基因的克隆及其新型表达载体的构建 |
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| 引用本文： | 霍丹群,范守城,张云茹,侯长军. phyA基因的克隆及其新型表达载体的构建[J]. 中国兽医科学, 2004, 34(3): 26-30 |
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| 作者姓名： | 霍丹群 范守城 张云茹 侯长军 |
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| 作者单位： | 1. 重庆大学,生物工程学院,教育部生物力学与组织工程重点实验室,重庆,400044
2. 重庆大学,生物工程学院,教育部生物力学与组织工程重点实验室,重庆,400044;重庆市养猪科学研究院,重庆市重点实验室,重庆,400246 |
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| 摘 要： | 直接以黑曲霉菌株F2 4 6基因组为模板 ,对植酸酶基因phyA的成熟肽 (分子长度为1347bp)进行了PCR扩增 ,再将PCR产物克隆入 pMD 18 T质粒 ,经DNA测序鉴定正确后 ,亚克隆入表达载体 pET30a 和 pMG36e ,成功构建了phyA基因 2种新型表达载体。植酸酶 phyA基因2种新型表达载体的构建 ,为进一步获得大量、高活性植酸酶以及研究和开发新型微生态制剂打下了基础。
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| 关 键 词： | 植酸酶 克隆 表达载体 黑曲霉 |
| 文章编号： | 1000-6419(2004)03-0026-05 |
| 修稿时间： | 2003-12-16 |
Cloning of phyA gene and construction of its new expression vectors |
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| HUO Dan-qun,FAN Shou-cheng. Cloning of phyA gene and construction of its new expression vectors[J]. Chinese Veterinary Science, 2004, 34(3): 26-30 |
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| Authors: | HUO Dan-qun FAN Shou-cheng |
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| Affiliation: | HUO Dan-qun~1,FAN Shou-cheng~ |
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| Abstract: | The phytase gene of Aspergillus niger (phyA) F246 strain, which had been already selected and identified by us in the key laboratory of biomechanics and tissue engineering, was amplified by polymerase chain reaction (PCR) in this study. The engineering strains JM109 (pET30a~( )-phyA) and JM109 (pMG36e-phyA) were constructed successfully after the PCR fragments were subcloned respectively into the expression vectors pET30a~ and pMG36e. The construction of new expression vectors for the phyA gene is the base of obtaining high active phytase and developing new microecological preparation. |
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| Keywords: | phytase cloning expression vector Aspergillus niger |
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