| 人血浆Fn的纯化、抗体制备及法医学应用的研究 |
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| 引用本文： | 李凡,张其英,陈忆九,赵子琴,吴文庆,顾云菊. 人血浆Fn的纯化、抗体制备及法医学应用的研究[J]. 中国法医学杂志, 1994, 0(4) |
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| 作者姓名： | 李凡 张其英 陈忆九 赵子琴 吴文庆 顾云菊 |
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| 作者单位： | 上海医科大学法医学系!200032，洛阳医学高等专科学校法医专业，上海医科大学法医学系!200032，上海医科大学法医学系!200032，上海医科大学法医学系!200032，上海医科大学法医学系!200032，上海医科大学法医学系!200032 |
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| 摘 要： | 本文用Gelatin－Senharose4B亲合层析法提纯人血浆Fn抗原，免疫家兔，收集抗血清，经过去Fn血浆制成的免疫固相吸附剂处理后，制备特异性抗人Fn血清。此抗血清1：200稀释，用于免疫组化PAP、DAB染色法，观察人头皮枪弹创、头皮挫裂创、腹部皮肤切创各4例创缘区Fn含量、分布变化的研究，能反映创线区Fn变化的规律，为法医学创伤的研究，进一步开展定量分析和定量测定，作了抗体准备。
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| 关 键 词： | 亲合层析 纤维联接蛋白（Fn） 抗Fn血清 |
ISOLATION AND PURIFICATION OF HUMAN FIBRONECTIN,PREPARATION OF ANTISERA AND ITS PRACTICAL USE |
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| Li Fan,Zhang Qiyin,Chen Yiju,Zhao Ziqin,Wu Wenqing,Gu Yunju. ISOLATION AND PURIFICATION OF HUMAN FIBRONECTIN,PREPARATION OF ANTISERA AND ITS PRACTICAL USE[J]. Chinese Journal of Forensic Medicine, 1994, 0(4) |
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| Authors: | Li Fan Zhang Qiyin Chen Yiju Zhao Ziqin Wu Wenqing Gu Yunju |
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| Affiliation: | Li Fan,Zhang Qiyin,Chen Yiju,Zhao Ziqin,Wu Wenqing,Gu YunjuDepartment of Forensic Medicine,Shanghai Medical Medical University 200032 |
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| Abstract: | The fibronectin was purified from human plasma by Gelatin-Sepharose 4B affinity chromatography. The rabbits were immunized with the fibronectin. the raw anti-fibronectin sera were absorbed bythe solid phase of the fibronectin free human serum. Then the specific anti-fibronectin sera were obtained. The fibronectins deposited on human bullet wounds, incised wounds as well as on blunt forceinJuries were demonst.ated by PAP immunohistochemical study using diluted anti-fibronectin serum.The results were quite sati factory. |
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| Keywords: | Affinity chromatography Anti-fibronectin serum Injury |
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