氨基胍对内毒素血症肉鸡肝自由基损伤的影响

为了探讨自由基在肉鸡内毒素血症发病机理中的作用,将90只30日龄的肉鸡,公母各半,随机分为生理盐水对照组(A组)、内毒素注射组(B组)和氨基胍治疗组(C组),每组30只。分别在试验后第1、3、5、7和9 h每组各宰杀6只,提取肝组织,检测肝组织中的总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性以及丙二醛(MDA)含量的变化。结果显示,内毒素血症时肝组织中SOD、GSH-Px和CAT活性降低,MDA含量明显升高,肝组织中T-AOC下降,而氨基胍治疗组的情况明显好转。证实,由自由基增多而引起的脂质过氧化损伤在肉鸡内毒素血症发病机理中起着重要的作用,氨基胍可有效降低脂质过氧化造成的损伤。

Effect of aminoguanidine on liver free radical injury in broilers with endotoxemia

To explore the effect of free radical in the pathogenesis of endotoxemia in broilers,ninety 30-day old broilers were randomly divided into three groups of A,B and C with 30 each.Group A was the control with injection of normal saline solution.Groups B and Group C were intravenously injected with lipopolysaccharides from Salmonella typhimurium but Group C was intravenously injected with amino-guanidine one hour before injection of endotoxin.Six broiler livers were collected from every group.Activities of T-AOC,T-SOD,GSH-Px and CAT and content of MDA in the liver tissue were examined at different time points post-treatment.Content of MDA of Group B was increased gradually,and activities of T-AOC,T-SOD,CAT and GSH-Px of Group B were decreased gradually.The activities of antioxidase of Group C were improved much better than Group B.The results indicated that lipid perioxidation of liver tissue induced by endotoxin plays an important role in endotoxemia in broilers,and aminoguanidine can reduce this injury and effectively protect liver functions and structure.