| 3种方法联合运用提取人体脱落上皮细胞DNA |
| |
| 引用本文： | 郭燕霞,陈松,刘开会. 3种方法联合运用提取人体脱落上皮细胞DNA[J]. 法医学杂志, 2009, 25(1): 42-43. DOI: 10.3969/j.issn.1004-5619.2009.01.014 |
| |
| 作者姓名： | 郭燕霞 陈松 刘开会 |
| |
| 作者单位： | 公安部物证鉴定中心,北京,100038 |
| |
| 摘 要： | 目的寻求提高法医物证检验中附有人体脱落细胞载体检材的DNA检出率的方法。方法根据案件检材的具体条件．选择3种提取方法即Chelex-100法、Chelex-100联合有机法和Chelex-100联合磁珠法。结果采用了分步提取DNA法，可充分、有效地提取微量DNA，提高了人体脱落上皮细胞有效DNA的检出率。结论根据第一步提取方法的检测结果，来调整下一步的提取策略，如是否加以纯化浓缩，可增加人体脱落上皮细胞有效DNA的检出率。
|
| 关 键 词： | 法医遗传学 提取法 脱落上皮细胞 |
The Application of Three Methods Combined for Extracting DNA from Human Epithelium |
| |
| GUO Yan-xia,CHEN Song,LIU Kai-hui. The Application of Three Methods Combined for Extracting DNA from Human Epithelium[J]. Journal of Forensic Medicine, 2009, 25(1): 42-43. DOI: 10.3969/j.issn.1004-5619.2009.01.014 |
| |
| Authors: | GUO Yan-xia CHEN Song LIU Kai-hui |
| |
| Affiliation: | GUO Yan-xia,CHEN Song,LIU Kai-hui (Forensic Science Institute of Public Security Ministry,Beijing 100038,China) |
| |
| Abstract: | Objective To improve DNA extraction from human epithelium. Methods Chelex-100, Chelex-100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case. Results With the stepwise extracting protocol, the trace amount of DNA was ana-lyzed most efficiently and the success rate of DNA extracting was better improved. Conclusion The effi- ciency of DNA extraction from human epithelium could be greatly improved by modifying the extracting protocol such as pu... |
| |
| Keywords: | forensic genetics extraction exfoliated epithelial cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
