Studies on ABH antigen grouping of ammoniacal extracts of bloodstains by absorption-elution |
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| Authors: | R E Gaensslen H C Lee S S DeGraw J Carroll-Reho |
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| Affiliation: | 1. Forensic Science Laboratories, University of New Haven, West Haven, CT 06516 U.S.A.;2. Connecticut State Police Forensic Science Laboratory, Meriden, CT 06450 U.S.A.;3. Department of Pathology, Yale University School of Medicine, New Haven, CT 06520 U.S.A.;1. Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt;2. Department of Biochemistry, National Research Centre, Cairo, Egypt;3. Department of Pharmacology and Toxicology, Faculty of Pharmacy, October 6 University, 6 October City, Giza, Egypt;4. Department of Medical Biochemistry, National Research Centre, Cairo, Egypt;5. Genetics and Cytology Department, National Research Centre, Cairo, Egypt;6. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo, Egypt;7. Department of Radiology, University of Missouri, Columbia, MO 65212, USA;8. Institute of Green Nanotechnology, University of Missouri, Columbia, MO 65212, USA;9. Centre for Advanced Materials and Industrial Chemistry, School of Applied Sciences, Health Innovation Research Institute, RMIT University, Australia;10. Research Reactor, University of Missouri, Columbia, MO 65212, USA;11. Department of Physics, University of Missouri, Columbia, MO 65212, USA;12. Department of Bioengineering, University of Missouri, Columbia, MO 65211, USA;1. Faculty of Computing, Universiti Teknologi Malaysia, Johor, Malaysia;2. School of Informatics and Applied Mathematics, Universiti Malaysia Terengganu, Terengganu, Malaysia;1. Department of Pharmaceutical Inspection, Ministry of Health, Ismailia 41522, Egypt;2. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt;3. Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Tabuk, Tabuk, Saudi Arabia;4. Department of Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt;5. Department of Pathology, College of Veterinary Medicine, King Faisal University, Saudi Arabia;6. Department of Physiology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt;7. Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt |
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| Abstract: | The effects of absorption time, titer of antiserum or lectin in the absorption stage, and elution temperature on relative antibody or lectin yield in elutes in the absorption-elution procedure were studied in bloodstains and ammoniacal extracts of bloodstains using conventional grouping reagents. In addition, monoclonal anti-A and anti-B and affinity-purified Ulex europaeus agglutinin I (UEA I) reagents were employed for comparison in these processes. Ammoniacal extracts of bloodstains and dried bloodstains on cotton substrata behaved comparably with respect to the parameters studied. The monoclonal anti-A and anti-B and UEA I reagents studied yielded satisfactory results, comparable in some cases to conventional reagents, with respect to the parameters studied. |
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