SPE—GC／MS、GC／NPD法检测血液中苯丙胺类毒品

目的利用GC／MS、GC／NPD与固相萃取（SPE）技术相结合，建立血液中苯丙胺类毒品的定性定量分析方法。方法采用Bond—ElutCerti{y固相柱、甲醇淋洗、二氯甲烷／异丙醇／氨水（78／20／2）洗脱固相萃取分离提取，比较了不同PH体系、稀释状态、洗脱溶剂对提取回收率的影响，建立血液中苯丙胺类毒品的GC／MS、GC／NPD定性定量分析方法。结果以GC／NPD分析AM、MA、MDA和MDMA浓度在15ng／mL-2000ng／mL、10ng／mL～1600ng／mL、20ng／mL-3000ng／ml、20ng／mL-3000ng／mL范围内线性关系良好，AM、MA、MDA和MDMA的检测限分别为10ng／mL、8ng／mL、15ng／mL、15ng／mL，方法平均回收率大于85％，标准偏差小于5％，GC／MS-Scan检测限分别为40ng／mL、32．0ng／mL、60．0ng／mL、60．0ng／mL。结论此方法可满足苯丙胺类毒品滥用者的血液定性定量分析。

Determination of amphetamine-Type Stimulants in blood by solid-phase extraction using gas chromatography/ mass spectrometry and gas chromatography/nitrogen-phosphor detector Technique

Objective To develop a qualitative and quantitative analysis method for the determination of amphetamine-type stimulants in blood samples using solid-phase extraction combined with GC/MS and GC/NPD.Methods The samples were extracted by solid-phase extraction with Bond-Elut Certify cartridge.Analytes were washed with methanol,and eluted by dichloromethane/isopropyl alcohol/ammonium hydroxide(78/20/2,v/v/v) mixed solvent.The optimum conditions for extraction,such as pH,dilution and elution conditions were stu...