| 伪狂犬病病毒Min-A株gE基因的克隆及序列分析 |
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| 引用本文： | 胡涛,崔保安,王学斌,杨明凡,张素梅,王岩. 伪狂犬病病毒Min-A株gE基因的克隆及序列分析[J]. 中国兽医科学, 2003, 33(8): 15-19 |
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| 作者姓名： | 胡涛 崔保安 王学斌 杨明凡 张素梅 王岩 |
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| 作者单位： | 1. 河南农业大学,畜牧兽医工程学院,河南,郑州,450002
2. 郑州牧业高等专科学校,河南,郑州,450008 |
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| 摘 要： | 参考GenBank中伪狂犬病病毒 (PRV) gE基因的序列设计了 1对引物 ,对PRVMin A株进行了PCR扩增 ,扩增产物克隆于 pGEM TEasy载体。对重组质粒进行限制性内切酶分析和基因测序 ,证实了克隆片段的可靠性。测序结果表明 ,目的片段包含 1个 174 0bp的开放性阅读框(ORF) ,编码由 5 79个氨基酸组成的多肽。同源性分析表明 ,PRVMin A株与PRVEa株、SH株gE基因的核苷酸同源性分别为 99.2 %、98.7% ;推导氨基酸的同源性分别为 98.6 %、97.2 %。
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| 关 键 词： | 伪狂犬病病毒 gE基因 克隆 序列分析 |
| 文章编号： | 1000-6419(2003)08-0015-05 |
| 修稿时间： | 2003-04-03 |
Cloning and sequence analysis of the gE gene of pseudorabies virus Min-A strain |
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| HU Tao ,CUI Bao-an ,WANG Xue-bing ,YANG Ming -fan ,ZHANG Su-mei ,WANG Yan. Cloning and sequence analysis of the gE gene of pseudorabies virus Min-A strain[J]. Chinese Veterinary Science, 2003, 33(8): 15-19 |
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| Authors: | HU Tao CUI Bao-an WANG Xue-bing YANG Ming -fan ZHANG Su-mei WANG Yan |
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| Affiliation: | HU Tao 1,CUI Bao-an 1,WANG Xue-bing 1,YANG Ming -fan 1,ZHANG Su-mei 1,WANG Yan 2 |
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| Abstract: | A pair of primers was designed according to the seq ue nces pulished by the GenBank in order to amplify gE gene of the pseudorabies virus Min-A strain.The gE gene was obtained by pol ymerase chain reaction, and then cloned into the pGEM-T Easy vector.The recombinant plasmid of pGTE-E was identified by restriction enzyme analysis an d sequencing ,Which proved completely its validity.Nucleotide sequenci ng revealed that this fragment contained an open reading frame of 1 740 bp en coding a 579 aa protein . The homology of the nucleotide sequence of PRV Min -A strain gE with PRV Ea strain,SH strain was 99.2% and 98.7%,respectively ;and the homology of predicted amino acids among them was 98.6% and 97.2%,respectiv ely. |
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| Keywords: | pseudorabies virus gE gene clonin g sequence analysis |
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