A Study of Leucine Aminopeptidase as a Possible Means of Identifying Semen |
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| Affiliation: | 1. Mahasarakham University, Maha Sarakham, Thailand;2. University of Hyderabad, Hyderabad, India;3. Asian Institute of Technology (AIT), Klong Luang, Thailand;4. Kalasin University, Kalasin, Thailand;1. Biochemistry and Nutrition Division, ICAR-Central Institute of Fisheries Technology, Cochin 682029, Kerala, India;2. Department of Food and Nutrition, Mody University, Lakshmangarh 332311, Rajasthan, India;3. College of fisheries, Central Agricultural University, Tripura 799210, India |
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| Abstract: | Leucine aminopeptidase (LAP) activity was investigated in a number of human body fluids, vegetable extracts, casework stains and vaginal swabs by means of either a spot test or photometric assay. The vaginal swabs were also examined for acid phosphatase (AP) and the levels of activity of both enzymes were compared. The results show that LAP can be readily detected in semen stains by means of the spot test or photometric assay. The spot test for LAP was found to be slower and less sensitive than the widely used AP test although no positive reactions were obtained with semen-free vaginal swabs. None of the other body fluids tested, including saliva, gave positive reactions although a slight positive reaction with faecal material was recorded. |
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