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| 国产试剂盒Goldeneye~(TM)20A在亲权鉴定中的应用评估 | |
| 引用本文： | 王洁,焦章平,黄艳梅,张庆霞,张新宁,唐晖,刘雅诚,冀晗. 国产试剂盒Goldeneye~(TM)20A在亲权鉴定中的应用评估[J]. 中国法医学杂志, 2012, 27(3): 205-208 |
| 作者姓名： | 王洁 焦章平 黄艳梅 张庆霞 张新宁 唐晖 刘雅诚 冀晗 |
| 作者单位： | 1. 新乡医学院法医物证学教研室,河南新乡453003;北京市公安局法医检验鉴定中心,北京100192 2. 北京市公安局法医检验鉴定中心,北京,100192 3. 新乡医学院法医物证学教研室,河南新乡,453003 4. 中国人民公安大学,北京,100038 |
| 基金项目： | 新乡医学院高学历人才启动基金(2005) |
| 摘 要： | 目的评估GoldeneyeTM20A试剂盒在亲权鉴定中的应用价值。方法应用GoldeneyeTM20A试剂盒对289宗亲权鉴定案例中的FTA卡血样本基因组DNA进行PCR扩增,扩增产物用ABI 3130xl遗传分析仪进行毛细管电泳,GeneMapper v3.2和GeneMarker HID软件进行基因分型及统计学分析,并与IdentifilerTM、SinofilerTM、PowerPlex16 3种试剂盒进行比较。结果采用GoldeneyeTM20A试剂盒,累积非父排除概率(CPE)为0.999 999 996,累积个人识别能力(CPD)达0.999 999 999 999 999 999 999 932 44,与目前常用的3种试剂盒相比较,GoldeneyeTM20A试剂盒在不排除的案例中具有更高的CPI值;在排除的案例中具有更多的排除指标。结论国产GoldeneyeTM20A试剂盒在亲权鉴定中有较高的应用价值。 |
| 关 键 词： | 法医物证学 GoldeneyeTM20A试剂盒 短串联重复序列 亲权鉴定 评估 |
Application and evaluation of the domestic GoldeneyeTM 20A kit in forensic paternity testing |
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| Wang Jie , Jiao Zhangping , Huang Yanmei , Zhang Qingxia , Zhang Xinning , Tang Hui , Liu Yacheng , Ji Han. Application and evaluation of the domestic GoldeneyeTM 20A kit in forensic paternity testing[J]. Chinese Journal of Forensic Medicine, 2012, 27(3): 205-208 | |
| Authors: | Wang Jie Jiao Zhangping Huang Yanmei Zhang Qingxia Zhang Xinning Tang Hui Liu Yacheng Ji Han |
| Affiliation: | 1.Department of Forensic Genetics in School of Basic Medical Science,Xinxiang Medical University,Henan Xinxiang 453003,China;2.Forensic Medical Identification Centre of Beijing Public Security Bureau,Beijing 100192,China;3.Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China) |
| Abstract: | Objective To evaluate the application value of the domestic GoldeneyeTM 20A kit in forensic paternity testing.Methods The genomic DNA from blood FTA samples of 289 paternity testing cases was amplified by the GoldeneyeTM 20A kit.The amplified products were detected by capillary electrophoresis,and the genotypes of 20 genetic markers were analyzed by GeneMapper v3.2 and GeneMarker HID Software.The genotyping results were compared with that of three common international kits including IdentifilerTM,SinofilerTM and PowerPlex16.Results With the GoldeneyeTM 20A kit,the cumulative probability of exclusion(CPE) and the cumulative power of discrimination(CPD) reached to 0.999 999 996 and 0.999 999 999 999 999 999 999 932 44,respectively.Compared with three kits above mentioned,higher combined paternity index(CPI) in non-exclusion cases and more excluding indicators in exclusionary cases were shown in this kit.Conclusion Compared with three imported kits,the domestic GoldeneyeTM 20A kit owns higher application value in forensic paternity testing. |
| Keywords: | forensic biological evidence GoldeneyeTM 20A kit short tandem repeat(STR) paternity testing evaluation |
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