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| 可卡因对小鼠离体脾细胞功能的影响 | |
| 引用本文： | 孙文平,江南,卢延旭,朱晓凯. 可卡因对小鼠离体脾细胞功能的影响[J]. 中国法医学杂志, 2012, 27(4): 265-267,271 |
| 作者姓名： | 孙文平 江南 卢延旭 朱晓凯 |
| 作者单位： | 1. 泰山医学院病理教研室,山东泰安,271000 2. 温州市公安局瓯海区分局,浙江温州,325000 3. 中国刑警学院法医病理教研室,辽宁沈阳,110035 |
| 基金项目： | 国家十五重点科技攻关课题(2001BA801B04) |
| 摘 要： | 目的 观察盐酸可卡因对小鼠体外培养脾细胞功能的影响.方法 采用小鼠脾细胞体外培养的方法,在细胞培养液中加入终质量浓度分别为10、20和100μg/mL的盐酸可卡因,连续培养.检测脾细胞的ConA增殖反应、IL-2、IFN-γ的活性.结果 实验组ConA诱导的小鼠脾细胞增殖反应明显降低,IL-2和IFN-γ的活性显著下降,与对照组相比较有显著性差异(P＜0.05;P ＜0.01).结论 可卡因对体外培养脾细胞功能有显著的抑制作用. |
| 关 键 词： | 法医毒理学 可卡因 脾细胞 体外培养 |
In vitro effects of cocaine on splenocyte function |
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| Sun Wenping,Jiang Nan,Lu Yanxu,Zhu Xiaokai. In vitro effects of cocaine on splenocyte function[J]. Chinese Journal of Forensic Medicine, 2012, 27(4): 265-267,271 | |
| Authors: | Sun Wenping Jiang Nan Lu Yanxu Zhu Xiaokai |
| Affiliation: | 1.Department of Pathology,Taishan Medical College,Shandong Taian 271000,China;2.Ouhai Branches of Wenzhou Municipal Public Security Bureall,Zhejiang Wenzhou 325000,China;3.Department of Forensic Toxicology,China Criminal Police College,Shenyang 110035,China) |
| Abstract: | Objective To examine the effects of Cocaine on splenocyte function in vitro.Methods The mouse splenocytes were cultured.Cocaine hydrochloride were added into the cell culture at the final concentration of 10,20 and 100 μg/mL,and then continuously cultured.The cell proliferative responses,activities of IL-2 and IFN-γ in supernatants were assayed.Results Compared with those of the control,ConA-induced splenocyte proliferative reactions and the supernatant IL-2,IFN-γ activities were all reduced significantly(P<0.05;P<0.01).Conclusion Cocaine hydrochloride may inhibit the mouse splenocyte function in vitro. |
| Keywords: | forensic toxicology cocaine splenocyte culture in vitro |
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