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浮游生物PCR检测及其在溺死鉴定中的研究进展
引用本文:张又川,王会品,胡孙林,苏会芳,赵建,刘超.浮游生物PCR检测及其在溺死鉴定中的研究进展[J].中国法医学杂志,2012,27(3):209-211.
作者姓名:张又川  王会品  胡孙林  苏会芳  赵建  刘超
作者单位:1. 中山大学中山医学院法医学系,广东广州,510080
2. 中山市公安局,广东中山,528403
3. 广州市刑事科学技术研究所,广东广州,510030
4. 中山大学中山医学院法医学系,广东广州510080;广州市刑事科学技术研究所,广东广州510030
基金项目:公安部重点研究计划项目(20102DYJGDST017)
摘    要:溺死鉴定是法医学中的重点和难点之一,目前在相关法医学案件中,主要采用法医病理学尸检与传统硅藻检验等方法综合分析进行鉴定。传统硅藻检验存在灵敏性较低,易于污染等不足。采用PCR法检测尸体不同脏器组织中是否存在水中浮游生物的DNA标记,适用范围广,信息量丰富,灵敏性和特异性较高,有较好的法医学应用前景,有望成为鉴定溺死的新方法。本文对相关研究进展进行综述,为相关研究和实践参考。

关 键 词:法医病理学  溺死  浮游生物  PCR

Advances in the studies on detection of planktons by PCR and its application to diagnosis of drowning
Zhang Youchuan , Wang Huipin , Hu Sunlin , Su Huifang , Zhao Jian , Liu Chao.Advances in the studies on detection of planktons by PCR and its application to diagnosis of drowning[J].Chinese Journal of Forensic Medicine,2012,27(3):209-211.
Authors:Zhang Youchuan  Wang Huipin  Hu Sunlin  Su Huifang  Zhao Jian  Liu Chao
Institution:1,3/1.Department of Forensic Medicine,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou 510080,China;2.Public Security Bureau of Zhongshan,Guangdong Zhongshan 528403,China;3.Guangzhou Forensic Science Institute,Guangzhou 510030,China)
Abstract:The diagnosis of drowning is one of the most important tasks and difficult issues in forensic science.Nowdays,investigation of death from drowning largely depends upon comprehensive analysis of autopsy findings in combination with detection of planktons in organs by traditional method in forensic practice.The traditional method for detection of diatoms is susceptible to contamination with relative low sensitiveness.Detection of plankton DNA by PCR can provide with abundant information with high sensitiveness and specificity,which has a wide applicability to diagnosis of drowning.In the present article,related progresses are reviewed as references to investigation of cases suspicious of drowning.
Keywords:forensic pathology  death by drowning  microorganism  PCR
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