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| 福州晋安河水域浮游微藻种群18SrDNA分析 | |
| 引用本文： | 张书田,卞戈,黄和鸣,陈乃宁,李军,刘泓,郑武.福州晋安河水域浮游微藻种群18SrDNA分析[J].中国法医学杂志,2012,27(3):197-200. |
| 作者姓名： | 张书田 卞戈 黄和鸣 陈乃宁 李军 刘泓 郑武 |
| 作者单位： | 1. 福州市刑事科学技术研究所,福建福州350003;福建农林大学功能基因组学研究中心,福建福州350002 2. 福州市刑事科学技术研究所,福建福州,350003 3. 福建农林大学功能基因组学研究中心,福建福州350002;福建农林大学资源与环境学院,福建福州350002 |
| 基金项目： | 福建省自然科学基金(2008J0305,2011J01389);中国博士后科学基金(20100480709) |
| 摘 要： | 目的应用分子生物学方法分析浮游微藻18SrDNA序列,分析福州晋安河浮游微藻种群分布。方法在晋安河分段设立11个点采集和水样本,采用优化CTAB法提取水样总DNA,通过PCR技术扩增并对浮游微藻18SrDNA序列进行测序,应用NCBI数据库序列进行比对筛选,分析该河段浮游微藻分布情况。结果筛选出67条浮游微藻序列,11个采样点部分藻类分布存在差异,常见种群为硅藻门藻类占40%,绿藻门藻类占12%,金藻门藻类占43%,原生动物类占5%,其中硅藻和金藻为优势种群。结论晋安河福州市河段内浮游微藻种群分布有一定特征,浮游微藻18SrDNA序列分析可望成为法医学溺水死亡鉴定的新方法。 |
| 关 键 词： | 法医病理学 溺死 浮游微藻 18SrDNA |
Population studies of planktonic microalgae 18SrDNA in Fuzhou inland rivers |
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| Zhang Shutian , Bian Ge , Huang Heming , Chen Naining , Li Jun , Liu Hong , Zheng Wu.Population studies of planktonic microalgae 18SrDNA in Fuzhou inland rivers[J].Chinese Journal of Forensic Medicine,2012,27(3):197-200. | |
| Authors: | Zhang Shutian Bian Ge Huang Heming Chen Naining Li Jun Liu Hong Zheng Wu |
| Institution: | 1,2/1.Fuzhou Criminal Science & Technology Institute,Fuzhou 350003,China;2.The Center of Fungal Genetics,Fujian Agricultrure and Forestry University,Fuzhou 350002,China;3.College of Resources and Environment,Fujian Agriculture and ForestryUniversity,Fuzhou 350002,China) |
| Abstract: | Objective In this study,the method of molecular biology was applied to analysis the 18SrDNA sequences of planktonic microalgae in Fuzhou Jinan rivers to research the spread of planktonic microalgae species.Methods In order to analysis the spread of planktonic microalgae species,Jinan rivers were segmented into eleven sections,and each sample was collected individually from them.The total DNA of water samples were extracted by CTAB method and the 18SrDNA sequences of planktonic microalgae were amplified and sequenced by polymerase chain reaction(PCR).The NCBI database sequence was applied to make compare and the right choice.Results 67 sequences of the planktonic microalgae were selected,there were differences in spread among 11 samples.The Bacillariophyta,Chlorophyceae,Chrysophyceae and protozoan took up 40%,12%,43% and 5% individually.The Bacillariophyta and Chrysophyceae were dominant species among them.Conclusion The spread of planktonic microalgae species in the section of Jinanriver across Fuzhou showed certain features,which may lead the analysis of 18SrDNA sequences of planktonic microalgae to a new way to identify forensic drowning deaths. |
| Keywords: | forensic pathology drowning planktonic microalgae 18SrDNA |
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