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| 免疫磁珠技术和精子抗原研究进展与法医学应用 | |
| 引用本文： | 郭磊,刘晓芳,韩莹,王燕,赵兴春,叶健. 免疫磁珠技术和精子抗原研究进展与法医学应用[J]. 中国法医学杂志, 2012, 27(3): 212-214,218 |
| 作者姓名： | 郭磊 刘晓芳 韩莹 王燕 赵兴春 叶健 |
| 作者单位： | 1. 中国人民公安大学,北京,100038 2. 北京市公安局昌平分局,北京,100740 3. 威海市公安局,山东威海,264200 4. 公安部物证鉴定中心,北京,100038 |
| 摘 要： | 免疫磁珠技术在生物学领域应用广泛,通过制备精子特异性抗体,并与磁性微球偶联,可达到对精阴混合斑中精子和女性阴道上皮细胞分离。本文简述了免疫磁珠技术的研究进展及磁珠主要制备方法,同时结合精子表面抗原的研究进展,提出磁珠抗体法提取精子DNA的可能性,并展望了磁珠抗体结合技术在法医学应用前景。 |
| 关 键 词： | 法医物证学 免疫磁珠 磁珠抗体结合技术 精子抗原 精子DNA 应用 |
Recent development of the immunomagnetic beads technology and sperm antigen research and their application in forensic science |
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| Guo Lei , Liu Xiaofang , Han Ying , Wang Yan , Zhao Xingchun , Ye Jian. Recent development of the immunomagnetic beads technology and sperm antigen research and their application in forensic science[J]. Chinese Journal of Forensic Medicine, 2012, 27(3): 212-214,218 | |
| Authors: | Guo Lei Liu Xiaofang Han Ying Wang Yan Zhao Xingchun Ye Jian |
| Affiliation: | 1.China People’s Public Security University,Beijing 100038,China;2.Changping Branches of Beijing Municipal Public Security Bureau,Beijing 100740,China;3.Weihai Municipal Public Security Bureau,Shandong Weihai 264200,China;4.Institute of Forensic Science Ministry of Public Security,Beijing 100038,China) |
| Abstract: | The technique of immunomagnetic beads have been widely used in the field of biology.Immunomagnetic beads coupling with spermatozoal-specific antibody can be applied to separate sperms from mixed stains.The paper primarily reviews recent development in immunomagnetic beads technology and preparation methods of magnetic beads.With recent researches on sperm antigens,the probability of extracting spermatozoal DNA by the method of immunomagnetic beads is put forwarded.And it is also prospected applications of the technique combining magnetic beads with antibodies in the field of forensic science. |
| Keywords: | forensic biological evidence immunomagnetic bead method of magnetic bead binding anti-gen spermatozoa antigen spermatozoa DNA application |
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