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| GoldeneyeTM 20A-M试剂盒在数据库建设中的应用 | |
| 引用本文： | 李秋阳,金萍,沈红缨,于蛟,郭飞,姜先华. GoldeneyeTM 20A-M试剂盒在数据库建设中的应用[J]. 中国法医学杂志, 2012, 27(4): 272-274 |
| 作者姓名： | 李秋阳 金萍 沈红缨 于蛟 郭飞 姜先华 |
| 作者单位： | 1. 辽宁省刑事科学技术研究所,辽宁沈阳,110032 2. 基点认知技术(北京)有限公司,北京,100190 3. 中国医科大学法医学院,辽宁沈阳,110002 |
| 基金项目： | 公安部应用科技创新计划项目(2005YYCXLNST107) |
| 摘 要： | 目的 研究GoldeneyeTM 20A-M试剂盒在DNA数据库建设中的应用.方法 针对血滤纸特性,优化不同条件血痕样品的取样量、扩增循环次数,缩短PCR扩增时间,以建立适合批量样品直接快速扩增检验的方法,并将其应用于1 200份数据库样品的检验中.结果 确定了批量样本检验的最适取样量和扩增循环次数,建立了快速扩增反应程序,1 200份样品的直接快速扩增检验成功率为98.4％,批量检验92份样品(1板)从取样到电泳完成只需6h.结论 GoldeneyeTM 20A-M试剂盒的应用,免去DNA提取过程,方法简单、快速、获得信息量大,适用于DNA数据库建设的需要. |
| 关 键 词： | 法医物证学 DNA数据库 直接扩增法 GoldeneyeTM 20A-M试剂盒 |
Study and application of GoldeneyeTM 20A-M kit in DNA database |
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| Li Qiuyang,Jin Ping,Shen Hongying,Yu Jiao,Guo Fei,Jiang Xianhua. Study and application of GoldeneyeTM 20A-M kit in DNA database[J]. Chinese Journal of Forensic Medicine, 2012, 27(4): 272-274 | |
| Authors: | Li Qiuyang Jin Ping Shen Hongying Yu Jiao Guo Fei Jiang Xianhua |
| Affiliation: | 1/1.Liaoning Institute of Criminal Science and Technology,Shenyang 110030,China;2.Peoplespot Inc.,Beijing 100190,China;3.China Medical University School of Forensic Medicine,Shenyang 110002,China) |
| Abstract: | Objective To study the application of GoldeneyeTM 20A-M kit in forensic DNA database.Methods The punch size and thermal cycling parameters were optimized according to the characteristics of the bloody filter paper,then a direct and rapid amplification method was established,which had been applied to 1 200 blood samples.Results The optimum punch size and thermal cycling parameters were determined.The success rate of 1 200 samples amplified by the established method was proved to be 98.4%.It only took 6 hours to finish testing 92 samples(a panel).Conclusion The GoldeneyeTM 20A-M kit,without the process of DNA extraction,is much more simple,rapid and effective,and can meet the needs of the forensic DNA database construction. |
| Keywords: | forensic biological evidence DNA database direct amplification GoldeneyeTM 20A-M kit |
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