| 潍坊地区六种常见嗜尸性蝇类mtDNA COI区序列研究 |
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| 引用本文： | Wang XJ,Wang XH,Diao LJ,Lu GP. 潍坊地区六种常见嗜尸性蝇类mtDNA COI区序列研究[J]. 法医学杂志, 2006, 22(2): 93-94 |
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| 作者姓名： | Wang XJ Wang XH Diao LJ Lu GP |
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| 作者单位： | 1. 潍坊市公安局,山东,潍坊,261061
2. 山东省公安厅,山东,济南,250001 |
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| 摘 要： | 目的解决蝇类成虫、蛹和蛆快速、准确鉴定的难题。方法随机采集潍坊地区人尸周围环境中的蝇类成虫、蛹和蛆,提取其mtDNA,经PCR扩增、产物检测与纯化、测序等,比较其序列差异。结果六种常见嗜尸性蝇类相互之间序列差异明显,同一种蝇的成虫、幼虫、蛹之间未见差异。结论mtDNACOI区序列分析可作为法医学嗜尸性蝇类种属鉴别的可靠方法。
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| 关 键 词： | 嗜尸性蝇类 种属鉴别 线粒体DNA细胞色素氧化酶辅酶I 法医学 |
| 文章编号： | 1004-5619(2006)02-0093-02 |
| 修稿时间： | 2005-02-25 |
Identification of six species of sarcosaphagous flies (Diptera) by sequence analysis of cytochrome oxidase subunit I gene (COI) in Weifang |
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| Wang Xin-Jie,Wang Xue-Hai,Diao Li-Jiang,Lu Gui-Ping. Identification of six species of sarcosaphagous flies (Diptera) by sequence analysis of cytochrome oxidase subunit I gene (COI) in Weifang[J]. Journal of Forensic Medicine, 2006, 22(2): 93-94 |
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| Authors: | Wang Xin-Jie Wang Xue-Hai Diao Li-Jiang Lu Gui-Ping |
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| Affiliation: | Public Security Bureau of Weifang municipality, Weifang 261061, China. |
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| Abstract: | Objective To identify sarcosaphagous flies and their larvae, pupa. Methods sarcosaphagous flies and their larvae, pupas were collected from human corpses and their surroundings in the Weifang city. A 304 bp region in COI gene was analyzed by mtDNA sequencing. Results The studied region showed no sequence divergence within same species and significant difference were found between different species in all samples. Conclusion It is a practical approach to identify these Sarcosaphagous flies and their larvae, pupas by sequence analysis of the 304 bp region of the COI in mtDNA. |
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| Keywords: | Sarcosaphagous flies species identification mtDNA COI forensic |
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