豚鼠脑震荡实验模型的建立和形态观察

实验用健康豚鼠46只,予以编号。用暴力撞击头部,建立脑震荡的动物模型。30只动物出现昏迷,于数分钟內恢复正常,无感觉和运动后遗症。分别于3、6、12、24、48、72h,1、2、3、4周处死。肉眼观察脑表面无异常,光镜和电镜下,72h以内各组胼胝休和脑干传出传入纤维轴索肿胀成串珠状或断裂成节段形,髓鞘崩解。其中24、48h有数量不等的巨噬细胞浸润;银染色可见轴索断端有收缩球。大脑皮质神经细胞部分有缺血固缩或肿胀变性,尼氏体溶解及卫星现象。血管反应及胶质细胞增生,偶见结节。伤后1～4周反应逐渐减轻。其余16只动物有不同部位的脑组织挫伤。对照组7只动物无上述阳性发现。

AN EXPERIMENTAL MODEL OF CEREBRAL CONCUSSION GUINEA, PIG AND THE MORPHOLOGICAL CHANGES

An experimental model of cerebral concussion induced by blunt force stroke in 46 guinea pigs was established. 30 out of 46 animals were unconscious immediately after stroke followed by recovery Within few minutes without any seuqel in the posttraumatic period The animals were killed at 3,6,12,24,48 and 72 hours, 1,2,3,4 weeks after injury. Within 72 hours after injury, some of axons of both the afferent and the efferent nerve fibers of the corpus callosum and the brain stem were swelling like necklace or disrupted into segments macrophage infiltration, glosis, satellitosis, pyknosis of nuclioli of neuron, lysis of nissles bodies, vascular reaction as well as the retraction of the torn ends of axon as demonstrated by the silver stain were observed in animals killed 24 and 48 hours after injury. The reaction mentioned above became milder and milder 1～4 weeks after injury. Brain contusion were produced in other 16 experimental animals. In 7 animals of control group, no change was observed