DNATyper^TM15基因座的研究与选择

目的为研发复合扩增荧光检测试剂盒,对现有的STR基因座进行分析研究并优选新的高鉴别力基因座。方法收集汉族、锡泊族、畲族、壮族、藏族等5个民族群体血样共1200份,提取DNA,应用复合扩增方法检测1200名5个民族群体无关个体的24个基因座的等位基因分布。结果TPOX和TH01基因座的等位基因在5个民族群体中分布不平衡;D2S1338、D6S1043和Penta E等3个STR基因座在5个民族群体中均具有高度遗传多态性,等位基因频率分布均匀,在各群体间无显著差异,而且等位基因传递遵循孟德尔遗传规律。结论确定出DNATyperTM15试剂盒中的14个适合中国人群体遗传学特征和法医学应用的STR基因座。

Study and selection of STR loci for DNATyperTM15 kit

In order to study and select new high polymorphic STR loci for human identity test,various STR loci were surveyed.As the result,DNATyperTM15 kit was developed.The kit allows simultaneous analyses of 15 genes including 11 CODISSTR loci,D3S1358,D5S818,D7S820,D8S1179,D13S317 D16S539,D18S51,D21S11,CSF1PO,FGA,vWA;three other STR loci Penta E,D6S1043,D2S1338 as well as a sex discriminating gene Amelogenin.1160 blood samples of five ethnic groups(Han,She,Xibo,Chuang and Tibetan) were analyzed.Two of the 13 CODIS loci(TPOX and TH01)were not included because of their imbalanced allele distribution in Chinese population.D6S1043,which was not included in two most commonly used commercial STR analysis kits,was shown to be an excellent STR marker for Chinese.D2S1338 and Penta E loci are highly polymorphic.Their allele distributions do not depart from Hardy-Weinberg expectation(HWE).There is no significant difference among the five ethnic groups.All loci of DNATyperTM15kit meet Hardy-Weinberg expectation.The DNATyperTM15 is suitable for genetic test of human identity for Chinese population.