| 基于高通量测序的多房棘球蚴感染小鼠肝组织中差异circRNA的生物信息学分析 |
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| 引用本文： | 李秀荣,李立,闫鸿斌,辇晓峰,张林生,付宝权,贾万忠. 基于高通量测序的多房棘球蚴感染小鼠肝组织中差异circRNA的生物信息学分析[J]. 中国兽医科学, 2020, 0(4): 437-443 |
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| 作者姓名： | 李秀荣 李立 闫鸿斌 辇晓峰 张林生 付宝权 贾万忠 |
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| 作者单位： | 中国农业科学院、兰州兽医研究所、家畜疫病病原生物学国家重点实验室、国家动物包虫病专业实验室、甘肃省动物寄生虫病重点实验室、农业部人兽共患病重点实验室;江苏省动物重要疫病与人兽共患病防控协同创新中心 |
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| 基金项目： | 国家重点研发计划项目(2018YFC1602504,2017YFD0501301);国家重点基础研究发展计划(973)项目(2015CB-150301);中央级公益性科研院所基本科研业务费专项(160312020016,1610312017001,1610312016012)。 |
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| 摘 要： | 分析多房棘球蚴感染小鼠肝组织中差异表达的环状RNA(circRNA),探究circRNA在宿主感染过程中可能参与的调控作用。采用高通量circRNA芯片技术比较感染组和对照组小鼠肝组织circRNA表达谱的差异,利用生物信息学对差异circRNA亲本基因进行GO和KEGG富集分析。结果显示,上调表达的circRNA有201个,下调表达的circRNA有9个,差异表达共计210个,经实时荧光定量PCR验证出其中4个上调和2个下调差异circRNA的表达水平变化趋势与芯片结果相一致。GO富集分析显示,在分子功能、生物过程及细胞组分均有富集;KEGG富集通路则有内吞作用、SNARE蛋白相关囊泡运动、矿物质吸收等与免疫相关的信号通路。结果提示,感染多房棘球蚴的小鼠肝组织与对照组小鼠肝组织比较,circRNA表达谱发生了显著性变化,这些差异表达的circRNA及其潜在的靶分子可能与多房棘球蚴感染宿主早期免疫反应有关。
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| 关 键 词： | 多房棘球蚴 小鼠 环状RNA 生物信息学 高通量测序 |
Bioinformatics analysis of differentially expressed circRNAs in hepatic tissues of mice infected with Echinococcus multilocularis larvae based on high-throughput sequencing |
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| LI Xiu-rong,LI Li,YAN Hong-bin,NIAN Xiao-feng,ZHANG Lin-sheng,FU Bao-quan,JIA Wan-zhong. Bioinformatics analysis of differentially expressed circRNAs in hepatic tissues of mice infected with Echinococcus multilocularis larvae based on high-throughput sequencing[J]. Chinese Veterinary Science, 2020, 0(4): 437-443 |
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| Authors: | LI Xiu-rong LI Li YAN Hong-bin NIAN Xiao-feng ZHANG Lin-sheng FU Bao-quan JIA Wan-zhong |
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| Affiliation: | (State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Zoonoses of Agriculture Ministry/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009,China) |
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| Abstract: | This study was designed to analyse differential expression of circular RNAs(circRNAs)in hepatic tissue of mice infected with Echinococcus multilocularis larvae,and to explore their possible regulatory function in infection development.The expression profiles of circRNAs and analysis of parental genes of differential circRNAs were carried out using high-throughput circ RNA microarray,and GO and KEGG enrichment,respectively.The results showed that 201 circRNAs were up-regulated while 9 were down-regulated among a total of 210 differentially expressed circRNAs.The results of real-time quantitative PCR confirmed that the expression trends of 4 up-regulated and 2 down-regulated differential circRNAs were consistent with the microarray results.GO analysis revealed that the differentially expressed circRNAs were enrichment in molecular functions,biological processes and cell components.And analysis of KEGG pathway indicated that they were involved in signaling pathways related to immunity,such as endocytic SNARE protein-related vesicle movement,mineral absorption,etc.In conclusion,this study suggested that circ RNA expression profiles of livers of mice infected with E.multilocularis significantly changed compared with those of control mice,inferring that the differentially expressed circRNAs and their potential target molecules may be involved in murine immuno-regulation during early stages of E.multilocularis larval infection. |
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| Keywords: | Echinococcus multilocularis larva mouse circular RNAs(circRNAs) bioinformatics high-throughput sequencing |
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