黄牛输卵管结构的组织学观察

采用冰冻切片HE染色方法对黄牛输卵管各部进行了组织学观察,以为生殖生理学和胚胎工程学等教学和研究工作提供基础资料。结果显示,黄牛输卵管伞和漏斗部为膜性结构,由两侧的假复层纤毛柱状上皮和中央的疏松结缔组织共同构成,没有肌层和外膜。壶腹部和峡部均由黏膜层、肌层和外膜构成。黏膜上皮为假复层纤毛柱状上皮,由柱状纤毛细胞、分泌细胞和基细胞共同构成,固有膜由疏松结缔组织构成,在峡部尚含有浆液性腺泡。黏膜层形成大小不等的初级纵行皱褶。漏斗部和壶腹部的大初级皱褶又分出数量不等的次级皱褶和三级皱褶;峡部只有初级皱褶,无次级皱褶和三级皱褶。肌肉层由内斜、外环两层平滑肌构成。外膜大部分为浆膜结构,在输卵管系膜侧为结缔组织的外膜。

Histological observation of structures of the Yellow cattle''''s oviduct

In order to provide basic materials for reproduction physiology and embryo engineering,the Yellow Cattle's oviduct was observed by freezing section and HE staining.The Yellow Cattle's oviduct umbrella and funnel were in membranous structure without tunica muscularis and adventitia,which consisted of psecudostratified ciliated columnar epithelium on both sides and loose connective tissue in the center.The oviduct ampulla and isthmus were composed of mucous layer,tunica muscularis and adventitia.Mucosae epithelium was psecudostratified ciliated columnar epithelium which consisted of ciliated columnar cells,secretory cells,and basilar cell,and lamina propria was loose connective tissues with serous gland in the oviduct isthmus.The mucosa layer had primary longitudinal folds,which derived of secondary and ter-tiary folds in the oviduct infundibulum and ampulla.There were only primary folds and no secondary or tertiary folds in the isthmus.The inner longitudinal and outer circular smooth muscles formed the tunica muscularis.Adventitia consisted mainly of serosa structure,but the connective tissue was at the oviduct propria side.It was concluded from the above results that there were basilar cells in the mucosae epithelium,besides ciliated columnar cells and secretory cells,and there were serous glands in the oviduct isthmus.