| 用双抗夹心间接ELISA方法检测p30确证人类精斑 |
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| 引用本文： | 苟清,侯一平,毛咏秋,吴梅筠. 用双抗夹心间接ELISA方法检测p30确证人类精斑[J]. 法医学杂志, 1991, 7(3): 10-13 |
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| 作者姓名： | 苟清 侯一平 毛咏秋 吴梅筠 |
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| 作者单位： | 华西医科大学法医学系，华西医科大学法医学系，华西医科大学法医学系，华西医科大学法医学系 |
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| 摘 要： | 检测p30确证精斑有下列几种方法:免疫扩散,免疫电泳,交叉免疫电泳,胶乳凝集抑制试验,薄层免疫分析和酶联免疫吸附试验(ELISA).这些方法各有优缺点,就灵敏度而论,则以
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| 关 键 词： | 双抗夹心间接ELISA方法 检测 p30 精斑 法医物证检验学 |
Determination of Human Seminal Stains by Sandwich Indirect ELISA |
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| Gou Qing Hou Yiping Mao Yongqiou Wu Meiyun. Determination of Human Seminal Stains by Sandwich Indirect ELISA[J]. Journal of Forensic Medicine, 1991, 7(3): 10-13 |
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| Authors: | Gou Qing Hou Yiping Mao Yongqiou Wu Meiyun |
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| Affiliation: | Gou Qing Hou Yiping Mao Yongqiou Wu MeiyunDepartment of Forensic SerologyWest China University of Medical Sciences |
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| Abstract: | For the purpose of confirming the human semen stains, a sandwich indirect ELISA test was established using the rabbite anti-human p30 serum and goat antihuman p30 serum. This method is sensitive enough to detect 200ng/ml p30. A blind test to examine 126 biological stains which have boon kept at room temperature for one year was carried out using this method.The test results showed 100% accuracy.There were not any false-positive and false-negative results. |
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| Keywords: | Enzyme-linked immunosorbent assay Human prostatespecific antigen p30 Conclusive test of semen stains The Project Supported by National Science Foundation of China |
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