| 应用MVR-PCR和银染法分析河北汉族人群D1S8基因座DNA结构多态性 |
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| 引用本文： | 丛斌,郭晓青,谷振勇,左敏,张国忠,姚玉霞,彭郁葱,王俊霞,尤红煜. 应用MVR-PCR和银染法分析河北汉族人群D1S8基因座DNA结构多态性[J]. 中国法医学杂志, 1999, 0(4) |
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| 作者姓名： | 丛斌 郭晓青 谷振勇 左敏 张国忠 姚玉霞 彭郁葱 王俊霞 尤红煜 |
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| 作者单位： | 河北医科大学法医学教研室!石家庄，050017，河北医科大学法医学教研室!石家庄，050017，河北医科大学法医学教研室!石家庄，050017，河北医科大学法医学教研室!石家庄，050017，河北医科大学法医学教研室!石家庄，050017，河北医科大学分子生物学研究室!石家庄，050017，河北医科大学分子 |
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| 摘 要： | 研究D1S8基因座重复序列内部的变异，并进行数字编码。应用MVR－PCR和聚丙烯酰胺梯度凝胶电泳银染法对240名河北汉人无关个体进行检测。结果每个个体得到约30个数字编码，未发现任何两无关个体所有编码相同，30个编码完全相同的概率为3．55×10－11。3种重复单位a-型、t-型、o-型出现的频率分别为54.77％、42．54％、2．69％。为小卫星变异重复序列的研究及其在法医实践中的应用提供了一种新方法。
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| 关 键 词： | 法科学 D1S8基因座 小卫星变异重复序列 数字编码 个人识别 |
Study on DNA polymorphism at D1S8 locus in Hebei Han population by using MVR-PCR and silver staining |
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| Cong Bin, Guo Xiaoqing, Gu Zhenyong,et al.. Study on DNA polymorphism at D1S8 locus in Hebei Han population by using MVR-PCR and silver staining[J]. Chinese Journal of Forensic Medicine, 1999, 0(4) |
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| Authors: | Cong Bin Guo Xiaoqing Gu Zhenyong et al. |
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| Affiliation: | Cong Bin, Guo Xiaoqing, Gu Zhenyong, et al. / |
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| Abstract: | For the purpose of studying the minisatellite variant repeat (MVR) sequences at D1S8 locus and obtaining digital codes, 240 unrelated individuals in Hebei Hanpopulation were detected by using MVR-PCR and Polyacrylamide gradient gel electrophoresisfollowed by silver staining. The result demonstrated that there are about 30 digita1 codes obtained. None of two unrelated individuals had the same code. The probability of identity of3O digital codes was 3. 55×10 -11. The percentage of three repeat units, a-type, t-type ando-type was 54. 77 %, 42. 54 %, 2. 69 % respectively. This approach provides a new methodfor the research of minisatellite variant repeat and its application to forensic science practice. |
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| Keywords: | Forensic science D1S8 locus Minisate1lite variant repeat Digital code Individual identificatidon |
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