Forensic analysis of soils using single arbitrarily primed amplification and high throughput sequencing |
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| Affiliation: | 1. School of Biological Sciences, Flinders University, Adelaide, South Australia, Australia;2. Forensic Science South Australia, Adelaide, South Australia, Australia;1. Laboratorio de Genética Molecular de Cruz Vital – Cruz Roja Ecuatoriana Quito, Ecuador;2. Laboratorio GENES Ltda, Medellin, Colombia;1. Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary;2. Research Centre for Natural Sciences of the HAS, Department of Complex Systems, Budapest, Hungary;1. Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany;2. IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal;3. Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain |
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| Abstract: | Soil is a remarkably complex, diverse, ubiquitous, and easily transferred material which can reveal highly useful information to assist forensic investigations. In spite of its potential usefulness, the use of genetic soil analysis appears to be currently underestimated in forensic practice. Herein we report on the use of single arbitrarily primed amplification followed by high throughput sequencing of DNA fragments for the comparison of soil samples. The composition and functional attributes of soil microbial communities from three different locations were compared and shown to be different based on the metagenomic sequencing data obtained. |
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| Keywords: | Forensic soil analysis Soil metagenomics High throughput sequencing Arbitrarily primed PCR |
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