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Results of Colombian exercise interlaboratory quality control 2012
Affiliation:1. Laboratorio GENES Ltda, Medellín, Colombia;2. Instituto de Biología, Universidad de Antioquia, Medellín, Colombia;3. Universidad CES, Medellín, Colombia;4. Universidad Pontificia Bolivariana, Medellín, Colombia;5. IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Portugal
Abstract:Participation in interlaboratory quality control exercises is one of the main mechanisms currently used for quality assurance and continuous improvement of the trials. The objective of this study was to design, to manage and to evaluate the Colombian Exercise Interlaboratory Quality Control 2012 (CEIQC-2012). The CEIQC-2012 included both practical and theoretical exercises. For practical exercise three samples were provided, two from blood and one from buccal swab, all on FTA cards, participants were requested to process the samples according to the methods and the markers used routinely in their own laboratories. For theoretical section four exercises were sent, and only one was mandatory, the remain have different degrees of difficulty and were optional. In the mandatory exercise, the participants were asked to calculate the partial and total IP of 15 autosomal STRs markers of an alleged father and a son. This exercise involved 28 laboratories from 6 Latin American and Caribbean countries (Brazil, Ecuador, Peru, Panama, Dominican Republic and Colombia), all reported results for the theoretical mandatory and 27 for the practical. Fifty-four STR markers distributed in autosomal, Y and X chromosomes were under consensus. The Proficiency Test conducted through the Colombian National Reference Laboratory has become a useful tool for quality assurance of all Colombian laboratories and some of Latin America and Caribbean that perform DNA testing to establish biological relationships. This exercise is also an excellent opportunity for constant experts training in the region.
Keywords:Quality control exercise  Interlaboratory comparison  Colombia  Latin America and the Caribbean  Genes Ltda
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