Forensic approach to analyzing rape cases |
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| Affiliation: | 1. Institute of Forensic Medicine, Criminology and Medical Deontology, School of Medicine, University, “St''s. Cyril and Methodius”, Skopje, The Former Yugolav Republic of Macedonia;2. INGEB, Sarajevo, Bosnia and Herzegovina;1. Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark;2. IPATIMUP–Institute of Molecular Pathology and Immunology of the University of Porto, Portugal;3. Faculty of Sciences of the University of Porto, Portugal;4. DNA Diagnostic Laboratory, State University of Rio de Janeiro, Brazil;1. Dipartimento di Scienze Biomediche e Sanità Pubblica, Sezione di Medicina Legale, Università Politecnica delle Marche, Ancona, Italy;2. Dipartimento di Sanità Pubblica, Neuroscienze, Medicina Sperimentale e Forense, Sezione di Medicina Legale e Scienze Forensi, Università di Pavia, Italy;3. Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Germany;4. Dipartimento di Scienze della Salute, Sezione di Medicina Legale, Università di Genova, Italy;1. DNAbiotec (Pty) Ltd, Pretoria, South Africa;2. ForPsych Consultants (Pty) Ltd, Perth, W.A., Australia;3. Forensic DNA Consultants (Pty) Ltd, Pretoria, South Africa;4. Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa |
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| Abstract: | With large number of criminal acts, such as rape and murder, identification of the perpetrator is very difficult to determine. The cause is the lack of sufficient quantity of biological traces provided as material evidence, or the biological material is decomposed and no result can be obtained by using other identification methods. Thus the case remains unsolved. The purpose of this research is to establish a reliable method for detecting semen presence in rape cases and to get DNA profile from the perpetrator of a crime. Vaginal swabs were taken using cotton swabs during gynecological examination or autopsy in 21 cases. The chemical detection of semen presence was performed using Phospathesmo Kits. The DNA extraction was performed using QIAAamp® DNA Mini Kit. The amplification was performed using AmpFistrIdentifiler Kit and AmpFistrYfiler Kit. The electrophoresis was performed using 310 ABI squenator. Results indicate that DNA profile was obtained in 4 cases where chemical tests did not prove semen presence using Phospathesmo Kits. In one case, neither semen presence was chemically proven nor DNA profile was obtained for autosomal STRs, but a profile for Y-STRs was obtained. Our analyses indicate that when the victim's body is examined within the first few hours or the first day, a genetic profile of the perpetrator of the criminal act is obtained. Besides using autosomal STRs, we recommend Y-STRs to be used in all rape cases, too, thus separating the male from female profile, and also the male kinship relatedness in cases of incest could be followed, the rape performed by several blood-related men or similar. |
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| Keywords: | Rape DNA Acid phospathase |
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