A 1-year time course study of human RNA degradation in body fluids under dry and humid environmental conditions |
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| Institution: | 1. Immunological Research, Universy of Cartagena, Cartagena, Colombia;2. Laboratorio GENES Ltda, Medellín, Colombia;3. Instituto de Biología, Universidad de Antioquia, Medellín, Colombia;4. Laboratorio de Genética Molecular, Cruz Roja Ecuatoriana, Quito, Ecuador;5. Universidad Pontificia Bolivariana, Medellín, Colombia;6. IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Portugal;1. Laboratorio de Genética Molecular de Cruz Vital – Cruz Roja Ecuatoriana Quito, Ecuador;2. Laboratorio GENES Ltda, Medellin, Colombia;1. Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany;2. IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal;3. Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain;1. Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary;2. Research Centre for Natural Sciences of the HAS, Department of Complex Systems, Budapest, Hungary |
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| Abstract: | Blood, saliva and semen are some of the forensically most relevant biological stains found at crime scenes. mRNA profiling is a reliable approach for the identification of the origin of an evidentiary trace. A stable set of markers and the knowledge about the effects of RNA degradation under different environmental conditions is necessary for the determination of an unknown biological stain. The aim of this work was to compare RNA degradation for human blood, semen and saliva at three different concentrations during a 1-year time period and exposed to dry and humid conditions. Also, this study addressed the question whether there are relevant differences in the efficiency of two RNA extraction methods. |
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| Keywords: | Forensic science Body fluid identification mRNA degradation DNA/RNA co-extraction Blood Saliva Semen |
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