Screening and identification of tissue-specific methylation for body fluid identification |
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| Affiliation: | 1. Laboratorio de Genética Molecular de Cruz Vital – Cruz Roja Ecuatoriana Quito, Ecuador;2. Laboratorio GENES Ltda, Medellin, Colombia;1. Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany;2. IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal;3. Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain;1. Armed Forces DNA Identification Laboratory, Armed Forces Medical Examiner System, United States;2. American Registry of Pathology, United States;3. Flinders University, Australia;4. National Institute of Standards and Technology, United States;1. IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Portugal;2. Faculty of Sciences of the University of Porto (FCUP), Portugal;3. Center of Biological and Natural Sciences, Federal University of Acre (UFAC), Rio Branco, AC, Brazil;4. DNA Diagnostic Laboratory (LDD), State University of Rio de Janeiro (UERJ), RJ, Brazil;1. School of Biological Sciences, Flinders University, Adelaide, South Australia, Australia;2. Forensic Science South Australia, Adelaide, South Australia, Australia;1. Department of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, PR China;2. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China |
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| Abstract: | Identification of body fluid stains can bring important information to crime case. Recent research in epigenome indicates that tissue-specific differentially methylated regions (tDMRs) show different DNA methylation profiles according to the type of cell or tissue, which makes it possible to identify body fluid based on analysis of DNA. This study screened and identified tDMRs from genome for forensic purpose. DNA samples from blood, saliva, semen, and vaginal fluid were analyzed by methylation sensitive represent difference analysis and Sequenom Massarray® quantitative analysis of methylation. Six blood-specific tDMRs were obtained. Two tDMRs display blood-specific hypomethylation, and four tDMRs show blood-specific hypermethylation. These tDMRs may discriminate blood stain from other body fluids. The result indicated that tDMRs could become potential DNA markers for body fluid identification. |
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| Keywords: | Body fluid identification DNA methylation tDMRs |
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