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Application of direct PCR in forensic casework
Affiliation:1. School of Biological Sciences, Flinders University, Australia;2. Forensic Science South Australia, Adelaide, Australia;1. Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Forensic Medicine, Laboratory of Forensic Genetics, “Sapienza” University of Rome, Viale Regina Elena 336, 00161 Rome, Italy;2. Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Histology and Medical Embryology, “Sapienza” University of Rome, Via A. Scarpa 16, 00161 Rome, Italy;3. Institute of Histology and Embryology, Catholic University of the Sacred Heart, Largo Francesco Vito 1, 00168 Rome, Italy;1. Forensic Science Program, Department of Applied Science, Prince of Songkla University, Hat Yai, Thailand;2. Police Forensic Science Center 10, Royal Thai Police, Thailand;3. Institute of Forensic Medicine, Royal Thai Police, Thailand;4. Central Police Forensic Science Division, Royal Thai Police, Thailand;1. Department of Public Health Sciences and Pediatrics, University of Turin, Turin, Italy;2. Human Genetics Foundation, HuGeF, Turin, Italy;3. Department of Public Health, University of Rome “Tor Vergata”, Rome, Italy;4. Department of Clinical and Biological Sciences, University of Turin, Turin, Italy;1. School of Biological Sciences, Flinders University, Australia;2. Forensic Science South Australia, Adelaide, Australia;1. Forensics Genetic and Biology Service, Central Delegation, National Institute of Legal Medicine and Forensic Sciences, I.P., Coimbra, Portugal;2. Faculty of Sciences, University of Lisbon, Lisbon, Portugal;3. CENCIFOR, Forensic Sciences Centre, Coimbra, Portugal
Abstract:Direct PCR is fast becoming a popular method in forensic science due to the advantages of saving time and money in the lab while increasing the probability of obtaining substantial results has a positive rippling effect. A laboratory is able to reduce the time spent on processing trace DNA samples, which can lead to investigators receiving important information in a timely manner that may not have been possible using standard methods. This study highlights the benefits of direct PCR in forensic casework by analysing trace and touch DNA on a range of substrates and exploring the loss of initial DNA due to extraction.
Keywords:Direct PCR  Latent DNA  Human identification  Short tandem repeat  Profiling
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