Rapid and sensitive typing of forensic stains by PCR amplification of polymorphic simple repeat sequences in case work. |
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Authors: | L Roewer J T Epplen |
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Institution: | Max-Planck-Institut für Psychiatrie, Martinsried, Germany. |
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Abstract: | After failure of conventional typing and multilocus DNA fingerprinting methods to compare a minute vaginal swab stain with blood from a murder victim and a suspect, we used enzymatic DNA amplification (polymerase chain reaction, PCR) to discriminate the DNAs by typing of simple sequence lengths polymorphisms. A mixed dinucleotide locus in the HLA-DRB gene region and three novel tetranucleotide polymorphisms located autosomally as well as on the human Y chromosome were used to exclude a jailed subject from a case of murder. |
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