A simplified procedure for simultaneous detection of salivary proteins and its application in paternity testing |
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| Authors: | J L Caeiro F Boán A Carracedo |
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| Affiliation: | 1. Department of Food Technology, School of Food Engineering, University of Campinas, Campinas, SP, Brazil;2. Department of Food Science and Human Nutrition, University of Illinois at Urbana—Champaign, IL, USA;3. Department of Food Science and Technology, State University of Londrina, Londrina, PR, Brazil;1. Department of Thoracic Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan 33305, Taiwan;2. Department of Computer Science and Information Engineering, National Taipei University, New Taipei City 23741, Taiwan;3. Institute of Imaging and Biomedical Photonics, National Chiao Tung University, Tainan 71150, Taiwan;4. Department of Thoracic Medicine, Chang Gung Memorial Hospital, Taoyuan 33378, Taiwan;5. Department of Respiratory Therapy, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan |
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| Abstract: | An alternative simplified procedure for the genetic analysis of salivary protein complex (SPC) which eliminates the need for a specific concentrated parotid sample is proposed. After the technique is applied isoelectric focusing and protein detection is carried out by silver staining. The chance of exclusion of non-fathers for these systems in our population is 0.161711 for Pr, 0.07948 for Db, 0.07836 for Pa and 0.00995 for PIF. The total exclusion rate for these systems is 0.30042. |
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