反相高效液相色谱法同时测定人血清中氯丙嗪与氯扎平的浓度

作者建立了同时测定人血清中氯丙嗪与氯扎平浓度的反相高效液相色谱(RP—HPLC)法。以利眠宁为内标,用环己烷—乙醚(1:1)混合溶剂提取血清中氯丙嗪和氯扎平。高浓度中毒者血清(>1ug/ml)则用乙腈沉淀后直接进行色谱测定。血清中氯丙嗪、氯扎平的方法回收率乎均各为98.5±4.0(SD)%,CV4.2%;99.9±4.1(SD)%,CV4.1%。两药在血清中的最低检测浓度为10ng/ml。本法应用于临床氯丙嗪、氯扎平合用药物的血浓度监测及司法中毒案例的分析,结果满意。

STUDIES ON RP-HPLC FOR THE SIMULTANEOUS DETERMINATIONS OF CHLORPROMAZINE AND CLOZAPINE IN HUMAN SERUM AND CLINICAL APPLICATIONS

A reversed-phase high performance liquid chromatographic method(RP-HPLC)was developed for the simultaneous determinations of human serum levels of chlopro-mazine and clozapine.Chlorpramozine and clozapine in serum was determined by adding chlordiazap-oxideas as an internal standard,extracting with cyclohexane-ether(1:1)(therapeutic evels)or precipitating by acetonitrile(Serum levels>1ug/ml),chromatographing ona 300×3.9mm 1.D.colomn packcd with u Bondapak C_(18)(10um),and detecting at UV254nm.The mobile phase was MeOH-H_2O-TEMED-HAC(65:35:0.3:0.52)The average recovery of the whole procedure were 99.9±4.1(SD)%.CV=4.1%for clozapine)98.5±4.0(SD)%,CV=4.2% for chlopromazine.The Minimum detetionlimit was 10ng/ml serum.The developed method was applied to clinical monitoring of serum levels from11 patients who wereco administered with multiple oral doses of clozapine and chlor-promazine.The serum concentrations of 0.150-0.445μg/ml and 0.014-0.086μg/ml werefound.Futhermore,one case of suicide was encountered.