A new pentaplex system to study short tandem repeat markers of forensic interest on X chromosome |
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| Authors: | Zarrabeitia María T Amigo Teresa Sañudo Carolina Zarrabeitia Ana González-Lamuño Domingo Riancho José A |
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| Affiliation: | Unit of Legal Medicine, Faculty of Medicine, University of Cantabria, Av. Herrera Oria, s/n 30911 Santander, Spain. zarrabet@unican.es |
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| Abstract: | A new method has been optimised to amplify five X chromosome short tandem repeat (STR) markers of interest in forensic medicine: human phosphoribosyl transferase (HPRTB), DXS101, androgen receptor (ARA), DXS7423 and DXS8377. Markers were conveniently amplified in a single PCR reaction with fluorochrome-labelled primers, which allowed the analysis of fragment sizes after injection into a capillary electrophoresis system. The most common alleles of each locus were sequenced and used in a control ladder to type unknown samples. |
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