| 气相色谱-串联质谱法分析尿和血中除草剂百草枯 |
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| 引用本文： | 张婷,谭家镒,齐宝坤,朱昱,姜兆林. 气相色谱-串联质谱法分析尿和血中除草剂百草枯[J]. 中国法医学杂志, 2009, 24(3): 161-163 |
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| 作者姓名： | 张婷 谭家镒 齐宝坤 朱昱 姜兆林 |
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| 作者单位： | 中国刑事警察学院,辽宁,沈阳,110035 |
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| 摘 要： | 目的建立尿和血中百草枯的离子交换固相萃取-气相色谱-串联质谱分析方法。方法尿样加内标乙基百草枯，用732阳离子交换树脂提取；血样加内标乙基百草枯，用三氯乙酸凝聚蛋白质后取上清液用732阳离子交换树脂提取。提取物用硼氢化钠在水溶液中碱性条件下还原，还原物用有机溶剂提取进行气相色谱-串联质谱法分析。结果尿和血中百草枯的提取率分别为76％和74％，检测限分别为2ng／mL和10ng／mL，尿添加百草枯100ng／mL和血添加百草枯500ng／mL水平的回收率分别为99．6±5．6％和99．3±7．6％（Mean±CV）。结论本文建立的分析方法灵敏度高，能够满足中毒致死案件检验及临床毒物检验的需要。
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| 关 键 词： | 法医毒物学 气质联用分析 固相萃取 百草枯 尿 血 |
Analysis of herbicide paraquat in urine and blood by gas chromatography coupling tandem mass spectrometry |
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| ZHANG Ting,TAN Jiayi,QI Baokun,ZHU Yu,JIANG Zhaolin. Analysis of herbicide paraquat in urine and blood by gas chromatography coupling tandem mass spectrometry[J]. Chinese Journal of Forensic Medicine, 2009, 24(3): 161-163 |
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| Authors: | ZHANG Ting TAN Jiayi QI Baokun ZHU Yu JIANG Zhaolin |
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| Affiliation: | ZHANG Ting,TAN Jiayi,QI Baokun,ZHU Yu,JIANG Zhoalin/China Criminal Police University,Shenyang 110035,China |
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| Abstract: | Objective A new method was developed to the analysis of paraquat in urine and blood by sold-phase extraction(SPE) using exchange resin and gas chromatography coupling tandem mass spectrometry (GC/MS/MS).Methods Urine samples were sticked with ethyl paraquat(internal standard,IS),extracted by SPE using 732 cation exchange resin.The blood samples were deproteined with trifluoroacetic acid and then were extracted by SPE using 732 cation exchange resin.The extracts were reducted under alkaline conditions in aqu... |
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| Keywords: | forensic toxicology GC/MS/MS solid-phase extraction paraquat urine blood |
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