Mini-STRs: A powerful tool to identify genetic profiles in samples with small amounts of DNA |
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| Authors: | V Lopes L Andrade M Carvalho A Serra F Balsa AM Bento L Batista C Oliveira F Corte-Real MJ Anjos |
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| Institution: | aForensic Genetic Service, Centre Branch, National Institute of Legal Medicine, I.P., Largo da Sé Nova 3000-213 Coimbra, Portugal;bCentre Branch, National Institute of Legal Medicine, I.P., Faculty of Medicine, University of Coimbra, Portugal |
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| Abstract: | Degraded human remains and crime scene evidences with small amounts of DNA typically reveal incomplete or null genetic profiles when using standard (large) STR amplicons. The technology of mini-STRs, using reduced-size STR amplicons, can help to recover information from these samples. In our Forensic Genetic Service several genetic profiles were obtained or completed using MiniFiler kit (Applied Biosystems) increasing the success rate in sample typing. In all studied cases no inconsistencies were found between profiles obtained with MiniFiler and Identifiler, suggesting that this mini-STR kit can be used to include low copy number (LCN) evidence profiles in STR databases. |
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| Keywords: | Mini-STR LCN Degraded samples Bones Teeth |
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