Validation of a microchip electrophoresis system as a DNA amplification control |
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Authors: | Bram Bekaert Monique Coomans Katleen Knaepen Lucie Larno Nathalie Thijs Elisabeth Vanhoutte Wim Van de Voorde Ronny Decorte |
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Affiliation: | aUZ Leuven, Department of Forensic Medicine, Laboratory of Forensic Genetics and Molecular Archaeology, Kapucijnenvoer 33, B-3000 Leuven, Belgium;bK.U.Leuven, Department of Human Genetics, Campus Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium |
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Abstract: | As part of the normal procedure in a forensic DNA laboratory, a quality control step of the amplified DNA is often implemented to ensure the correct amplification of the sample before it is analysed in downstream applications. A validation study was undertaken to investigate a new microchip electrophoresis system (MultiNa, Shimadzu Corporation) claiming high resolution and sensitivity compared to routine polyacrylamide gel electrophoresis (PAGE). An array of STR multiplexes (AmpFISTR™ SGM+, GenePrint® FFFL, PowerPlex™ 16, PowerPlex™ Y, an in-house Y-STR multiplex and AmpFISTR™ Profiler) was tested under both standard and low copy number PCR parameters to evaluate the accuracy, reproducibility and sensitivity of this technique. These tests showed that the microchip system did not have improved sensitivity compared to PAGE though had increased resolution and high reproducibility between samples. |
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Keywords: | MultiNa Microchip electrophoresis Amplification control |
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