HLA-A位点DNA分型及其法医学应用 |
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引用本文: | 刘利民,贾静涛,梁健,U.Gyllensten. HLA-A位点DNA分型及其法医学应用[J]. 中国法医学杂志, 1998, 0(4) |
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作者姓名: | 刘利民 贾静涛 梁健 U.Gyllensten |
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作者单位: | 中国医科大学法医血清学教研室!沈阳,110001,中国医科大学法医血清学教研室!沈阳,110001,中国医科大学第一临床学院器官移植科!沈阳,110001,瑞典乌普萨拉大学医学遗传学系 |
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摘 要: | 应用聚合酶链反应0寡核苷酸探针(PCR-SSO)斑点杂交技术,对222名辽宁地区汉族人群无关个体进行HLA-A基因检测,研究中国辽宁地区汉族群体的HLA-A座位基因分布状况。共检出HLA-A等位基因24个,其中以等位基因HLA-A0201最为常见,频率为0.2635;依次是2402101和1101,等位基因频率分别为0.1847和0.1262.理论杂合度为87%,个人鉴别机率为92%,非父排除率为73.3%。在中国辽宁汉族中检出73种基因型,对观察值和期望值进行X2检验,符合Hardy-Weinberg平衡定律(x2=6.28,df=9,0.5<P<0.75)。家系分析结果表明按照孟德尔方式遗传。提出的中国辽宁汉族HLA-A等位基因的遗传基因情况,可用于法医学个人识别和亲子鉴定。人类学,HLA相关疾病,及器官移植研究。
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关 键 词: | HLA-A基因分型 多态性 聚合酶链反应(PCR) 寡核苷酸探针 |
DNA typing of HLA-A locus and forensic application |
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Abstract: | The distribution of gene at HLA-A locus was studied in 222 Chinese Han POpulation, using polrmerase chain rea c t ion/ oligon ucleotide probe hybridizatiion technique.24 alleles were found. The most frequent alleIe was the * 0201 (0. 2635). The * 2402101 (0. 1847) and * 1101 (0. 1262) were the next. Consequently the heterozagosity was 87%, while the power of discrimination was 92%. The exclusion probability of paternity was 73.3%. 73 genotypes were detected. The observed genotype distribution conforms withthe expected under the Hardy-Weinberg equilibrium. The data base is useful for the study of HLA-disease association, paternity testing and individual identification as well as anthropology. |
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Keywords: | HLA-A genotyping Polymorphism Polymerase chain reaction (PCR) Oligonucleotide probe |
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