| 不同温度下铜绿蝇蛹期发育形态观察 |
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| 引用本文： | 王贺,王玲,李泽民. 不同温度下铜绿蝇蛹期发育形态观察[J]. 中国法医学杂志, 2008, 23(5): 324-326 |
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| 作者姓名： | 王贺 王玲 李泽民 |
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| 作者单位： | 河北医科大学基础医学院病原生物学教研室,河北,石家庄,050017 |
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| 摘 要： | 目的观察不同温度下铜绿蝇蛹形态变化特征规律并进行发育阶段划分。方法在16℃、20℃、24℃、28℃、32℃下饲养铜绿蝇，于化蛹起，每隔12h取样1次，直至半数羽化。解剖样本，在体视显微镜下观察其体表形态变化。结果铜绿蝇蛹形态发育过程包含Ⅰ蛹体分头、胸、腹；Ⅱ头咽骨贴壁；Ⅲ原头出现；Ⅳ呼吸角破壳；Ⅴ翅与足出现及褐化；Ⅵ鬃毛出现及褐化；Ⅶ复眼轮廓形成及色素化等7个时间标志性特征，并据此将蛹期划分为9个阶段。结论在已知现场环境温度的前提下，蝇蛹形态变化具有阶段性特征，以此划分蛹期阶段，可望作为推断死后间隔时间（PMI）的参考。
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| 关 键 词： | 法医昆虫学 死后间隔时间 形态变化 铜绿蝇 蛹期 |
Pupal morphogenesis of Lucilia cuprina in different constant temperatures and its significance in Forensic Medicine |
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| WANG He,WANG Ling,LI Ze-min. Pupal morphogenesis of Lucilia cuprina in different constant temperatures and its significance in Forensic Medicine[J]. Chinese Journal of Forensic Medicine, 2008, 23(5): 324-326 |
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| Authors: | WANG He WANG Ling LI Ze-min |
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| Affiliation: | (Department of Parasitology, Hebei Medical University of Basic Medicine, Shijiazhuang 050017, China) |
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| Abstract: | Objective To study the chronology of development within puparia to determine the postmortem interval(PMI).Methods The necrophagous fly Lucilia cuprina was reared successively in constant temperatures(16℃,20℃,24℃,28℃,32℃).Sample,dissecte,and observe the pupal morphologic changes with the stereomicroscope.Results 7 morphological changes within the puparium can be used as age-markers and the pupal period of Lucilia cuprina can be divided into 9 developmental stages.The time required for each pupal stage was inversely proportional to the temperature.Conclusion Pupal developmental stages can be used as reference to the estimation of PMI. |
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| Keywords: | Forensic entomology Postmortem interval Morphogenesis Lucilia cuprina Pupa |
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