| 常见嗜尸性甲虫及蝇类虫体不同部位mtDNA的提取效果 |
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| 引用本文： | Guo YD,Cai JF,Su RN,Chang YF,Lan LM,Li X,Wen JF. 常见嗜尸性甲虫及蝇类虫体不同部位mtDNA的提取效果[J]. 法医学杂志, 2010, 26(5): 336-339. DOI: 10.3969/j.issn.1004-5619.2010.05.005 |
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| 作者姓名： | Guo YD Cai JF Su RN Chang YF Lan LM Li X Wen JF |
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| 作者单位： | 1. 中南大学,基础医学院法医学系,湖南,长沙,410013
2. 中南大学,基础医学院法医学系,湖南,长沙,410013;中南大学,湘雅二医院心理中心,湖南,长沙,410011 |
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| 基金项目： | 国家自然科学基金资助项目,2007年度湖南省优秀青年骨干教师培养项目 |
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| 摘 要： | 目的探讨改良后十六烷基三甲基溴化铵(cetyltriethylammnonium bromide,CTAB)法对常见嗜尸性昆虫虫体不同部位mtDNA的提取效果。方法随机采集放置在呼和浩特地区室外草地家兔尸体上丽蝇科的丝光绿蝇[Lucilia sericata(Meigen)]、埋葬甲科的Nicrophorus fossor(Erichson)各13例,采用改良CTAB法分别提取每只昆虫头部、胸肌、腿部、翅膀4个部位的mtDNA,核酸蛋白测定仪检测DNA纯度及质量浓度,琼脂糖凝胶电泳检测其PCR产物,测定PCR产物序列,上传GenBank。结果 13例丝光绿蝇[Lu-cilia sericata(Meigen)]的胸肌均提取到了DNA,10例头部样本、6例腿部样本、4例翅膀样本得到DNA;13例Nicrophorus fossor(Erichson)胸肌DNA均提取成功,5例头部样本、8例腿部样本及3例翅膀样本提取出DNA。结论改良CTAB法可用于对嗜尸性昆虫胸肌及其他部位进行DNA的提取。
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| 关 键 词： | 法医遗传学 昆虫学 DNA,线粒体 嗜尸性昆虫 |
Comparison of mtDNA extraction from different parts of sarcosaphagous insects |
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| Guo Ya-Dong,Cai Ji-Feng,Su Ri-Na,Chang Yun-Feng,Lan Ling-Mei,Li Xiang,Wen Ji-Fang. Comparison of mtDNA extraction from different parts of sarcosaphagous insects[J]. Journal of Forensic Medicine, 2010, 26(5): 336-339. DOI: 10.3969/j.issn.1004-5619.2010.05.005 |
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| Authors: | Guo Ya-Dong Cai Ji-Feng Su Ri-Na Chang Yun-Feng Lan Ling-Mei Li Xiang Wen Ji-Fang |
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| Affiliation: | GUO Ya-dong1,CAI Ji-feng1,2,SU Ri-na1,CHANG Yun-feng1,LAN Ling-mei1,LI Xiang1,WEN Ji-fang1(1.Department of Forensic Medicine,School of Basic Medical Science,Central South University,Changsha 410013,China,2.Center of Psychology,Second Xiang-Ya Hospital,Changsha 410011,China) |
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| Abstract: | Objective To explore mitochondrial DNA(mtDNA) extraction effects of different parts from sarcosaphagous insects using improved cetyltriethylammnonium bromide(CTAB) method.Methods Thirteen Lucilia sericata(Meigen) and 13 Nicrophorus fossor(Erichson) were collected from the corpses of rabbits placed on the outdoor lawn in Huhehot district.Four parts(head,chest muscle,legs and wings) of insect were collected,and the mtDNA of all samples were extracted using CTAB method.The purity and concentration were tested ... |
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| Keywords: | forensic genetics entomology DNA mitochondrial sarcosaphagous insects |
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