Y染色体STR的银染复合扩增

目的建立一套Y染色体STR的复合扩增体系,检测中国藏族人群的单倍型分布。方法利用复合扩增的方法扩增DYS434、DYS443和DYS456三个基因座,利用聚丙烯酰胺凝胶电泳银染进行分型,检测西藏藏族101名无关男性个体单倍型分布。结果三个基因座在藏族样本中分别检测出4、4、6个等位基因,共检测出31种单倍型,其单倍型的变异度是0.9481,标准误为0.0049。结论Y-STR的复合扩增在法医学的亲权鉴定和个人识别中有重要的作用。

Distributions of haplotypes for three Y-STR loci in a Tibetan ethnic group of Chinese population by using Y-STR multiplexes

Objective One multiplex genotyping system was developed in using silver staining with allelic lad - ders for three Y-chromosome STR markers(DYS434,DYS443,and DYS456),with a view towards the applica - tion of rapid and simple genotyping assay methods for DNA profiling.The distributions of haplotypes for three Y-STR loci(DYS434,DYS443,and DYS456)was investigated in a Tibetan ethnic group of Chinese population.Methods Allele and haplotype frequencies at these Y-STRs loci(DYS434,DYS443,and DYS456)were analysed by PCR amplification using Y-STR multiplexes,followed by horizontal non-denaturing polyacrylamide gelelec-trophoresis in101unrelated males of Tibetan ethnic group in Lasa of China.Results A total of31different haplotypes were found,16of them being unique.The haplotype diversity value(which is the same as the dis - crimination index)calculated from all three loci combined was0.9481,which is informative.Conclution The Y-STR multiplexes provide useful information for forensic analysis and paternity tests and can also be of great ben - efit for providing information not normally available from autosomal DNA systems.