快速溶剂萃取法提取血中的有机磷类农药

目的建立生物检材血液中有机磷类农药的快速溶剂萃取方法。方法通过优化快速溶剂萃取各参数，提取血中的有机磷类农药，同时达到在线净化的效果，然后进行GC／FPD分析检测。结果血中5种常见有机磷类农药的平均回收率均在70％以上，在0．05μg／mL-5μg／mL浓度范围内线性关系良好，检测限（S／N=10）均低于20ng／mL。结论该方法优于其它传统提取方法，快速、简便、高效且操作自动化，可广泛应用于生物检材血液中有机磷类农药的检验鉴定。

Extraction of organophosphorus pesticides in human blood using accelerated solvent extraction method

Objective To develop accelerated solvent extraction methods for determining organophosphorus pesticides in blood sample. Methods Organophosphorus pesticides were extracted by accelerated solvent extraction method with optimized pa- rameters, and purified by the alumina powder online. Concentrated elution was analyzed with GC/FPD. Results The recoveries of organophosphorus pesticides were larger than 70~. A good linear relationship was obtained at the concentration range of 0.05μg/mL-5μg/mL. The limit of detection was prior than 20ng/mL for signal to noise ratio（S/N） being 10. Conclusion The proposed method can be used to extract organophosphorus pesticides in biological specimens due to its superiority comparing with other traditional extracting methods.