| 精子细胞定向捕获与分离技术初步研究 |
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| 引用本文： | 赵兴春,姜伯玮. 精子细胞定向捕获与分离技术初步研究[J]. 刑事技术, 2012, 0(1): 14-17 |
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| 作者姓名： | 赵兴春 姜伯玮 |
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| 作者单位： | 1. 公安部物证鉴定中心,北京,100038
2. 中国科学院上海应用物理研究所,上海,201800 |
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| 摘 要： | 目的探索并研究精子细胞定向捕获与分离技术，初步建立精子细胞特异性分离与DNA提取的方法与试剂体系。方法通过特异性定向捕获复合体（精子特异性抗体一磁性纳米微球）的制备，在一定的试剂体系环境下，实现精子细胞的定向捕获与分离。结果能够实现精子细胞的定向富集与分离，通过后续的提取过程，获得了高质量的DNA，并获得了相应的完整STR分型结果。
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| 关 键 词： | 精子细胞 定向捕获 特异性分离 DNA提取 STR |
Preliminary study on a high specific method for directional capture and separation of sperm cells from forensic samples |
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| ZHAO Xing-chun , JIANG Bo-wei. Preliminary study on a high specific method for directional capture and separation of sperm cells from forensic samples[J]. Forensic Science and Technology, 2012, 0(1): 14-17 |
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| Authors: | ZHAO Xing-chun JIANG Bo-wei |
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| Affiliation: | (Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China) |
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| Abstract: | Objective To explore the capture and separation technology of sperm cells and to establish a reagent system for sperm cell-specific separation and DNA extraction methods respectively.Methods The sperm samples were prepared with specific capture and separation system and cell were separation from cell mixtures.Results With the help of above complexes,sperm cells were isolated from different mixtures of sperm cells and buccal cells.The specificity of reagent system was additionally checked by DNA genotyping.A complete STR genotyping was obtained by the current reagent system for the capture and separation sperm cells from cell mixtures. |
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| Keywords: | sperm cells capture specific separation DNA extraction STR |
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