大鼠皮肤切创愈合过程中泛素的表达及其法医学意义

目的观察大鼠皮肤切创后泛素(ubiquitin)表达的变化规律。方法应用免疫组化方法,观察大鼠皮肤切创后1、3、6、12h和1、3、6、10、14d ubiquitin的表达情况,并用图像分析系统进行图像分析。结果正常对照组大鼠皮肤有低水平ubiquitin表达,切创后ubiquitin表达增加,伤后6d达峰值,伤后10d开始下降,伤后14d恢复到正常水平。结论ubiquitin可作为法医学损伤时间推断的有效指标。

The expression of ubiquitin in the healing process of incised wounds of rat's skin and its significance to forensic medicine

Objective To evaluate the changes of ubiquitin expression in incised wounds of the rat skin.Methods The testing rat groups were subjected to incised skin wounds,with normal rat skin used as control.The expression level of ubiquitin was assessed using immunohistochemistry and imaging analysis technique on skin samples taken at 1,3,6,12 h,and on day 1,3,6,10,and 14 d after injury.Results The expression level of ubiquitin was low in the skin of normal control group.Increased level of ubiquitin expression could be observed 1 h after injury.The expression level of ubiquitin reached its peak on day 6 and started to decline on day 10,and then returned to its normal level on day 14 d after injury.Conclusion Ubiquitin may serve as a potentially useful marker for forensic determination of the skin wound age.